14-ATC2. Engineering resistance to disease in pigs

14-ATC2。

• 批准号: BB/M018342/1
• 负责人: Christopher Whitelaw
• 金额: $ 12.19万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM018342%2F1
• 关键词: 14; ATC2.; Engineering; resistance; disease

This project addresses food security and environmental sustainability by increasing production efficiency by reducing wastage due to disease and thus decreasing the carbon footprint of pork production. We will target the significant impact of influenza virus on one of our core livestock industries. Swine influenza (SIV) ranks consistently among the top 3 economic diseases affecting breeding, nursery, and finishing herds. In addition, influenza is the top zoonotic viral disease of swine. Our strategy is based on the recent validation of a novel RNA decoy approach in chickens. We now aim to use this newly developed technology to produce genetically engineered (GE) pigs expressing novel RNA molecules designed to prevent replication and propagation of SIV. We aim to mitigate or even eliminate mortality and morbidity due to SIV infection in pigs, while additionally protecting human health by reducing the zoonotic flu pandemic potential. It is the intention that this project will function as a 'prototype' study and, if successful, with further method refinement lead to development of a commercial product.

该项目通过减少疾病造成的浪费，提高生产效率，从而减少猪肉生产的碳足迹，解决粮食安全和环境可持续性问题。我们会针对流感病毒对本港其中一个主要畜牧业的重大影响。猪流感（SIV）一直是影响育种、保育和肥育猪群的3大经济疾病之一。此外，流感是猪的头号人畜共患病毒性疾病。我们的策略是基于最近在鸡中验证的一种新的RNA诱饵方法。我们现在的目标是利用这项新开发的技术来生产基因工程（GE）猪表达新的RNA分子，旨在防止SIV的复制和繁殖。我们的目标是减轻甚至消除猪SIV感染引起的死亡率和发病率，同时通过降低人畜共患流感大流行的可能性来保护人类健康。该项目的目的是作为一个“原型”研究，如果成功的话，进一步的方法改进将导致商业产品的开发。

项目成果

Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.

• DOI: 10.1371/journal.ppat.1006206
• 发表时间: 2017-02
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Burkard C;Lillico SG;Reid E;Jackson B;Mileham AJ;Ait-Ali T;Whitelaw CB;Archibald AL
• 通讯作者: Archibald AL

Potential of gene drives with genome editing to increase genetic gain in livestock breeding programs.

• DOI: 10.1186/s12711-016-0280-3
• 发表时间: 2017-01-04
• 期刊: Genetics, selection, evolution : GSE
• 作者: Gonen S;Jenko J;Gorjanc G;Mileham AJ;Whitelaw CB;Hickey JM
• 通讯作者: Hickey JM

Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells.

• DOI: 10.1186/s40064-016-2536-3
• 发表时间: 2016
• 期刊: SpringerPlus
• 作者: He Z;Proudfoot C;Whitelaw CB;Lillico SG
• 通讯作者: Lillico SG

Genome Editing and the Future of Farming meeting report.

• DOI: 10.1007/s11248-016-0006-x
• 发表时间: 2017-04
• 期刊: Transgenic research
• 影响因子: 3
• 作者: Hoyos-Flight M;Brady E;Sang H;Whitelaw B
• 通讯作者: Whitelaw B

Pigs Lacking the Scavenger Receptor Cysteine-Rich Domain 5 of CD163 Are Resistant to Porcine Reproductive and Respiratory Syndrome Virus 1 Infection.

缺乏CD163的猪富含清道夫受体的富含半胱氨酸的结构域5具有对猪生殖和呼吸综合征病毒1感染的抗性。

• DOI: 10.1128/jvi.00415-18
• 发表时间: 2018-08-15
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Burkard C;Opriessnig T;Mileham AJ;Stadejek T;Ait-Ali T;Lillico SG;Whitelaw CBA;Archibald AL
• 通讯作者: Archibald AL