ROLE OF THE HIV-1 REGULATORY GENES IN TRANSGENIC MICE

描述：（改编自申请人摘要）研究者将 尝试将相关的HIV基因导入小鼠的生殖系， 获得转基因动物的意图， 用于研究HIV发病机制的模型。 提交人 四个具体目标，第一个是确定艾滋病毒的作用， 调节基因，达特和nef，在诱导发病机制，通过引入每个 它们单独或组合，进入小鼠的生殖系。 的 第二个目标是靶向特定的HIV调节基因，特别是 达特基因，以限制细胞类型在小鼠中使用组织特异性 转录控制元件，以增强对 预期的疾病表型。 第三个目标是试图理解 HIV潜伏和激活的机制，因为它涉及到相互作用 在病毒LTR（长末端重复序列）和病毒和细胞之间， 反式激活因子。 最后，调查员建议查明 环境因素的重要性，特别是其他人类病毒（如 如JC病毒和人肝炎B病毒），在诱导HIV发病中的作用。 转基因小鼠

DESCRIPTION: (Adapted from applicant's abstract) The investigator will attempt to introduce relevant HIV genes into the germline of mice with the intent of deriving transgenic animals which will serve as appropriate models for the study of mechanisms of HIV pathogenesis. The author has four specific aims, the first of which is to define the role(s) of the HIV regulatory genes, tat and nef, in inducing pathogenesis by introducing each of them individually, or in combination, into the germline of mice. The second goal is to target specific HIV regulatory genes, particularly the tat gene, to restricted cell types in mice by the use of tissue-specific transcriptional control elements in order to enhance the detection of anticipated disease phenotypes. The third goal is to try to understand the mechanism of HIV latency and activation as it pertains to the interaction between the viral LTR (long terminal repeat) and viral and cellular trans-activators. Lastly, the investigator proposes to ascertain the importance of environmental factors, particularly other human viruses (such as JC virus and human hepatitis B virus), in inducing HIV pathogenesis in transgenic mice.