DEVELOPMENT OF A PUBLIC GENE EXPRESSION DATABASE

公共基因表达数据库的开发

• 批准号: 6388335
• 负责人: GILBERT JAY
• 金额: $ 85.83万
• 依托单位: ORIGENE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6388335
• 关键词: Internet; computer human interaction; computer program /software; computer system design /evaluation; functional /structural genomics; gene expression; human data; human genetic material tag; informatics; information retrieval; molecular biology information system; nucleic acid sequence; online computer

The development of a comprehensive gene expression database is essential for the discovery of functions for the estimated 100,000 human genes. The EST (expressed sequence tag) approach to identify gene sequences has already led to the assembly of approximately 50,000 unigenes, transcriptional units defined by overlapping EST sequences. While DNA sequence information is of great importance, knowledge of where each gene is expressed and under what circumstances is an essential next step in functional discovery. In this fast-track SBIR application, OriGene Technologies Inc. proposes to determine the tissue-specific expression profiles of initially 2,000 human unigenes, using a relatively high- throughput approach, and to develop a public database with innovative on- line search capabilities to support the general biomedical research community. This effort is in response to our belief that most functional dissection of genes over the next decade will be by individuals seeking out target genes with specific expression profiles that befit certain developmental, differentiation or disease characteristics. A state-of-the-art gene expression database, that also serves as a one-stop reagent shopping site, will be of singular importance and will be a commercial success. PROPOSED COMMERCIAL APPLICATIONS: What allows the rapid progression to functional discovery of a gene has to be, apart from the DNA sequence, the delineation of its sites of expression. The development of an on-line gene expression database, which also serves as a one-stop shopping site for genomic research reagents, will have wide utilities among investigators in both academia and the private sector. Such a Web site will become a mainstay of the biotechnology industry and an assured commercial success.

开发一个全面的基因表达数据库对于发现大约10万个人类基因的功能至关重要。EST（表达序列标签）方法来识别基因序列已经导致约50，000 unigenes的组装，由重叠EST序列定义的转录单位。 虽然DNA序列信息非常重要，但了解每个基因在哪里表达以及在什么情况下表达是功能发现的重要下一步。 在这个快速通道SBIR应用中，OriGene Technologies Inc.建议使用相对高通量的方法确定最初2，000个人类单基因的组织特异性表达谱，并开发具有创新在线搜索能力的公共数据库，以支持一般生物医学研究社区。这一努力是对我们的信念的回应，即在未来十年中，大多数基因的功能解剖将由个体寻找具有适合某些发育，分化或疾病特征的特定表达谱的靶基因。一个最先进的基因表达数据库，也作为一个一站式的试剂购物网站，将是非常重要的，将是一个商业上的成功。拟议的商业应用：除了DNA序列之外，快速发现基因功能的关键还在于确定其表达位点。开发一个在线基因表达数据库，也可作为基因组研究试剂的一站式购物网站，将对学术界和私营部门的研究人员具有广泛的效用。这样一个网站将成为生物技术工业的支柱，并确保取得商业成功。