DEVELOPMENT OF A PUBLIC GENE EXPRESSION DATABASE

开发一个全面的基因表达数据库对于发现大约10万个人类基因的功能至关重要。EST（表达序列标签）鉴定基因序列的方法已经导致了大约50,000个unigenes的组装，这些转录单位由重叠的EST序列定义。虽然DNA序列信息非常重要，但了解每个基因在哪里表达以及在什么情况下表达是功能发现的重要下一步。在这个快速通道的SBIR应用程序中，OriGene技术公司建议使用相对高通量的方法确定最初2000个人类单一基因的组织特异性表达谱，并开发一个具有创新在线搜索功能的公共数据库，以支持一般生物医学研究界。我们相信，在未来十年，大多数基因的功能解剖将由个体寻找具有特定表达谱的靶基因，这些基因适合某些发育、分化或疾病特征。一个最先进的基因表达数据库，也可以作为一站式试剂购物网站，将具有独特的重要性，并将取得商业上的成功。建议的商业应用：除了DNA序列外，使基因快速发展到功能发现的因素必须是其表达位点的描绘。开发一个在线基因表达数据库，它也可以作为基因组研究试剂的一站式购物网站，将在学术界和私营部门的研究人员中具有广泛的效用。这样一个网站将成为生物技术产业的支柱，并确保商业上的成功。

The development of a comprehensive gene expression database is essential for the discovery of functions for the estimated 100,000 human genes. The EST (expressed sequence tag) approach to identify gene sequences has already led to the assembly of approximately 50,000 unigenes, transcriptional units defined by overlapping EST sequences. While DNA sequence information is of great importance, knowledge of where each gene is expressed and under what circumstances is an essential next step in functional discovery. In this fast-track SBIR application, OriGene Technologies Inc. proposes to determine the tissue-specific expression profiles of initially 2,000 human unigenes, using a relatively high- throughput approach, and to develop a public database with innovative on- line search capabilities to support the general biomedical research community. This effort is in response to our belief that most functional dissection of genes over the next decade will be by individuals seeking out target genes with specific expression profiles that befit certain developmental, differentiation or disease characteristics. A state-of-the-art gene expression database, that also serves as a one-stop reagent shopping site, will be of singular importance and will be a commercial success. PROPOSED COMMERCIAL APPLICATIONS: What allows the rapid progression to functional discovery of a gene has to be, apart from the DNA sequence, the delineation of its sites of expression. The development of an on-line gene expression database, which also serves as a one-stop shopping site for genomic research reagents, will have wide utilities among investigators in both academia and the private sector. Such a Web site will become a mainstay of the biotechnology industry and an assured commercial success.