TRANSCRIPTION CONTROL BY THE NUCLEAR ONCOPROTEIN, E1A
核癌蛋白 E1A 的转录控制
基本信息
- 批准号:3199775
- 负责人:
- 金额:$ 15.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1991
- 资助国家:美国
- 起止时间:1991-07-01 至 1994-06-30
- 项目状态:已结题
- 来源:
- 关键词:Adenoviridae DNA binding protein antibody binding proteins cell free system gene expression genetic transcription laboratory rabbit molecular cloning mutant oncoproteins phosphorylation protein sequence regulatory gene transcription factor viral carcinogenesis virus antigen virus genetics virus infection mechanism
项目摘要
The long term goal of this proposal is to elucidate the transcription
regulatory pathways that are also targets of oncogene action such as the
adenovirus ElA. Several oncogene products have been identified.
Determination of the biochemical functions of these oncogene products
will eventually elucidate the pathways that lead to transformation and
tumorigenesis. Evidence is accumulating that the majority of the
oncogene products are involved in the control of gene expression. This
proposal involves functional analysis Of ElA oncogene products with
regard to their effect on the activities on the cellular proteins such as
E2F, E4F and E2FBP. E2F and E4F are cellular transcription factors that
are involved in the control of the adenovirus early genes. E2FBP is an
E2F binding protein that modulates E2F activity by forming a complex with
E2F which in turn is regulated by ElA proteins.ElA dissociates E2F/E2FBP
complex requiring the domains 1 and 2 of ElA and these two domains are
also involved in the transformation function of this oncoprotein. Thus a
major focus of this proposal is biochemical characterization of E2FBP and
production of specific antisera against E2FBP which will help analyzing
the basis of ElA control. The SV40 T antigen and the HPV E7 protein
possess sequence homology with domains 1 and 2 of ElA and these two
oncoproteins also trans-activate adenovirus E2 promoter depending on the
E2F binding site. These two proteins will also be analyzed for their
ability to control E2F/E2FBP interaction. The other interest of this
proposal is to analyze the mechanism that control the DNA binding
activity of the ElA-regulated transcription factor E4F. Specific
reagents, like peptide antisera and cDNA clones, will be obtained using
the peptide sequence information. These reagents will be extremely
valuable in the studies of E4F regulation by phosphorylation as well as
by the adenovirus ElA gene products. The proposal also focuses on
identification of a cellular kinase that might be involved in the
regulation of E4F DNA binding activity.
该提案的长期目标是阐明转录
调控途径也是癌基因作用的目标,例如
腺病毒ElA。 已鉴定出几种癌基因产物。
确定这些癌基因产物的生化功能
最终将阐明导致转型和发展的途径
肿瘤发生。 越来越多的证据表明,大多数
癌基因产物参与基因表达的控制。 这
提案涉及 ElA 癌基因产物的功能分析
关于它们对细胞蛋白质活性的影响,例如
E2F、E4F 和 E2FBP。 E2F 和 E4F 是细胞转录因子
参与腺病毒早期基因的控制。 E2FBP是一个
E2F 结合蛋白通过与以下物质形成复合物来调节 E2F 活性:
E2F 又受 ElA 蛋白调节。ElA 解离 E2F/E2FBP
复合体需要 ElA 的域 1 和 2,这两个域是
也参与这种癌蛋白的转化功能。 因此一个
该提案的主要重点是 E2FBP 的生化特征和
生产针对 E2FBP 的特异性抗血清,这将有助于分析
ElA 控制的基础。 SV40 T 抗原和 HPV E7 蛋白
与ElA的结构域1和2具有序列同源性,并且这两个
癌蛋白还反式激活腺病毒 E2 启动子,具体取决于
E2F结合位点。 这两种蛋白质也将被分析其
控制 E2F/E2FBP 交互的能力。 这个的另一个兴趣
提议是分析控制DNA结合的机制
ElA 调节的转录因子 E4F 的活性。 具体的
试剂,如肽抗血清和 cDNA 克隆,将使用以下方法获得
肽序列信息。 这些试剂将非常
在研究 E4F 磷酸化调节以及
由腺病毒ElA基因产物。 该提案还重点关注
鉴定可能参与该过程的细胞激酶
E4F DNA 结合活性的调节。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Pradip Raychaudhuri其他文献
Pradip Raychaudhuri的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Pradip Raychaudhuri', 18)}}的其他基金
相似海外基金
Targeting pathogenic TAR DNA-binding protein 43 to treat frontotemporal dementia and motor neuron disease
靶向致病性 TAR DNA 结合蛋白 43 治疗额颞叶痴呆和运动神经元疾病
- 批准号:
nhmrc : 2001572 - 财政年份:2021
- 资助金额:
$ 15.25万 - 项目类别:
Ideas Grants
Electron microscopic analysis of a G4 DNA-binding protein Rif1, a key organizer of chromosomal domains
G4 DNA 结合蛋白 Rif1(染色体结构域的关键组织者)的电子显微镜分析
- 批准号:
18K06102 - 财政年份:2018
- 资助金额:
$ 15.25万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional analysis of methylated DNA-binding protein CIBZ in mouse embryogenesis
甲基化DNA结合蛋白CIBZ在小鼠胚胎发生中的功能分析
- 批准号:
16K08587 - 财政年份:2016
- 资助金额:
$ 15.25万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Continuous directed evolution of a light-controlled DNA-binding protein
光控DNA结合蛋白的连续定向进化
- 批准号:
437922-2013 - 财政年份:2015
- 资助金额:
$ 15.25万 - 项目类别:
Postgraduate Scholarships - Doctoral
Development of a photo-controlled DNA-binding protein
光控 DNA 结合蛋白的开发
- 批准号:
459937-2014 - 财政年份:2015
- 资助金额:
$ 15.25万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Doctoral
Function and evolution of mitochondrial DNA-binding protein in the fission yeast
裂殖酵母线粒体DNA结合蛋白的功能和进化
- 批准号:
15K07168 - 财政年份:2015
- 资助金额:
$ 15.25万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional analysis of the single-stranded DNA-binding protein FUBP1 as a transcriptional regulator of hematopoietic stem cell self-renewal
单链DNA结合蛋白FUBP1作为造血干细胞自我更新转录调节因子的功能分析
- 批准号:
276833671 - 财政年份:2015
- 资助金额:
$ 15.25万 - 项目类别:
Research Grants
Continuous directed evolution of a light-controlled DNA-binding protein
光控DNA结合蛋白的连续定向进化
- 批准号:
437922-2013 - 财政年份:2014
- 资助金额:
$ 15.25万 - 项目类别:
Postgraduate Scholarships - Doctoral
Structural ans functional analysis of single-stranded DNA-binding protein DdrA
单链 DNA 结合蛋白 DdrA 的结构和功能分析
- 批准号:
26506030 - 财政年份:2014
- 资助金额:
$ 15.25万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of a photo-controlled DNA-binding protein
光控 DNA 结合蛋白的开发
- 批准号:
459937-2014 - 财政年份:2014
- 资助金额:
$ 15.25万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Doctoral