COMPARATIVE ANALYSIS OF JUN PROTEIN FUNCTION

Jun蛋白功能对比分析

• 批准号: 3197591
• 负责人: KEVIN T RYDER
• 金额: $ 16.9万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3197591
• 关键词: cell growth regulation; cyclic AMP; gel electrophoresis; gene expression; genetic mapping; genetic promoter element; genetic regulation; genetic regulatory element; genetically modified animals; laboratory mouse; molecular cloning; molecular genetics; nucleic acid sequence; oncogenes; phorbols; polymerase chain reaction; protein structure function; scintillation counter; tissue /cell culture; transcription factor

The long term objective of the proposed research is to elucidate the role of the jun genes (c-jun, jun-B, and jun-D) in regulating cellular proliferation. Because little is presently known about the physiological consequences of jun gene expression, a broad approach is initially suggested. Specific objectives include comparing the transcriptional regulatory activity and oncogenic potential of the three Jun proteins. In addition, if functional differences manifest in these experiments, it is anticipated that it will be important to map the protein domains that contribute to differential activity because the information obtained may be useful in identifying the genes that Jun proteins regulate. Concomitantly, two approaches are described for studying the cellular and molecular consequences of jun gene expression. One strategy involves generating conditionally jun (-) cell lines through a regulable negatively complementing Jun protein. The other relies upon expression of a jun gene within its normal tissue compartments in a transgenic mouse strain. As this project develops, effort will be concentrated on the experiments that hold the most promise for identifying jun regulated genes.

拟议研究的长期目标是阐明其作用 jun 基因（c-jun、jun-B 和 jun-D）在细胞调节中的作用 增殖。 因为目前对生理学知之甚少 jun 基因表达的后果，最初是一种广泛的方法 建议。 具体目标包括比较转录 三种 Jun 蛋白的调节活性和致癌潜力。 在 此外，如果这些实验中出现功能差异，那就是 预计绘制蛋白质结构域图谱非常重要 有助于差异活动，因为获得的信息可能是 可用于识别 Jun 蛋白调节的基因。 与此同时， 描述了两种研究细胞和分子的方法 jun基因表达的后果。 一种策略涉及生成 通过可调节的负向条件性 jun (-) 细胞系 补充Jun蛋白。 另一种依赖于 jun 基因的表达 在转基因小鼠品系的正常组织区室中。 作为 这个项目的发展，努力将集中在实验上 最有希望鉴定jun调控基因。