COMPARATIVE ANALYSIS OF JUN PROTEIN FUNCTION

拟议研究的长期目标是阐明 jun基因（c-jun，jun-B和jun-D）在调节细胞凋亡中的作用 增殖 因为目前对生理学的了解很少， jun基因表达的后果，一个广泛的方法是初步 建议道。 具体目标包括比较转录 三种Jun蛋白的调节活性和致癌潜力。 在 此外，如果在这些实验中表现出功能差异， 预计绘制蛋白质结构域将是重要的， 有助于差异活动，因为获得的信息可能是 有助于识别Jun蛋白调节的基因。 同时， 描述了用于研究细胞和分子的两种方法， Jun基因表达的后果。 一种策略是 条件性jun（-）细胞系通过一个可调控的负 补充Jun蛋白。 另一种依赖于jun基因的表达 在转基因小鼠品系的正常组织区室中。 作为 这个项目的发展，努力将集中在实验， 最有希望用于鉴定Jun调控的基因。

The long term objective of the proposed research is to elucidate the role of the jun genes (c-jun, jun-B, and jun-D) in regulating cellular proliferation. Because little is presently known about the physiological consequences of jun gene expression, a broad approach is initially suggested. Specific objectives include comparing the transcriptional regulatory activity and oncogenic potential of the three Jun proteins. In addition, if functional differences manifest in these experiments, it is anticipated that it will be important to map the protein domains that contribute to differential activity because the information obtained may be useful in identifying the genes that Jun proteins regulate. Concomitantly, two approaches are described for studying the cellular and molecular consequences of jun gene expression. One strategy involves generating conditionally jun (-) cell lines through a regulable negatively complementing Jun protein. The other relies upon expression of a jun gene within its normal tissue compartments in a transgenic mouse strain. As this project develops, effort will be concentrated on the experiments that hold the most promise for identifying jun regulated genes.