PRODUCTION OF TAXOL BY PLANT TISSUE CULTURE

通过植物组织培养生产紫杉醇

• 批准号: 3199544
• 负责人: JOHN AYNSLEY
• 金额: $ 29.62万
• 依托单位: ESCAGENETICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 1994-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3199544
• 关键词: Conifera; antineoplastics; bioreactors; biosynthesis; biotechnology; biotransformation; diterpenes; drug design /synthesis /production; fluorescence; genotype; growth media; high performance liquid chromatography; mass spectrometry; medicinal plants; microtubules; neoplasm /cancer pharmacology; paclitaxel; plant extracts; plant genetics; plant growth /development; plant growth regulators; radiotracer; thin layer chromatography; tissue /cell culture; tubulin

The overall purpose of the investigation is to develop an economic process for the production of taxol, or functionally similar compounds, in bioreactors using plant tissue culture. Taxol is an antitumor drug derived primarily from Taxus brevifolia, the Western Yew. It has excellent confirmed activity against cancer, particularly refractory human ovarian cancers. T. brevifolia is very slow growing and sufficiently rate to seriously limit the supply of the drug. Plant cell and tissue culture has the potential to provide a controlled supply of the drug to meet the needs of clinical trials and subsequent demand if approved for general clinical use. The proposed work is intended to develop the knowledge base in technical areas that are critical to economic bioreactor production of taxol. Key areas of the investigation are selection of high taxane producing cells, prevention of taxane catabolism, protection of cells from any adverse effects of taxanes and mitigation of any metabolic rate limiting steps. The work will be completed within an applied framework that uses bioengineering scale-up criteria to determine areas needed for improvement. In the short-term, the work will form an important foundation for independent programs in development of tissue culture protocols and scale-up in bioreactors. In the longer term, the investigations will identify strategies for induction of taxol biosynthesis and productivity improvements.

调查的总体目的是发展经济