ANIHWA call2: Sustainable management of aquaculture fish health through the development of low cost plant-derived vaccines

ANIHWA 呼吁 2：通过开发低成本植物源疫苗实现水产养殖鱼类健康的可持续管理

• 批准号: BB/M027856/1
• 负责人: George Lomonossoff
• 金额: $ 29.88万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM027856%2F1
• 关键词: ANIHWA; call2; Sustainable; management; aquaculture

The aquaculture industry is the fastest growing food-producing sector worldwide. To achieve a sustainable aquaculture food-fish production and manage the aquaculture fish health, vaccination has been found to be an effective method for increasing economic output and improving animal welfare. It has also permitted the development of more sustainable fish farming with reduced use of antibiotics and chemicals. Since viral diseases pose a serious threat to aquacultures worldwide, this lack of preventive measures has extensive negative consequences. For instance, pancreas disease (PD), a viral fish disease caused by the Salmonid alphavirus (SAV) has a significant impact on Norwegian salmonid aquaculture as well as in other European countries e.g. UK. Another fish viral disease, viral haemorrhagic septicaemia (VHS), which can affect most species of salmonid fish reared in fresh water or sea water, is caused by the VHS virus. The economic loss caused by VHSV on farmed rainbow trout is a challenging task in Finnish aquaculture. Outbreaks of VHShave also occurred in Norway and the UK. VHSV has been detected in marine fish, and is known to have high mutation rate and adaptability. Thus, there is an urgent need to develop low-cost fish vaccines to assist sustainable aquaculture production in Europe. Thus, we three Europeans partners (Norway, UK and Finland) aim to advance our existing plant-based recombinant protein production technologies to establish a transnational plant-based low cost vaccine production platform to facilitate the future production of fish vaccines in large quantity at low cost. We have selected PD and VHS as two cases for proof-of-principle and will test our three different tobacco-production technologies (i.e. transient expression by producing virus like particles, chloroplast genetic engineering and tobacco BY-2 cell line platform). By comparing the three systems in terms of total production costs, scaling-up potential and timeline by the end of the project period, the PlantVac project can provide a technology-based plant production platform for future production of PD and VHS vaccines as well as other fish vaccines. The PlantVac consortium consists of experienced scientists with the required expertise on three of the proposed plant production systems, fish health, veterinary immunology and economics.

水产养殖业是全球增长最快的食品生产部门。为了实现水产养殖食用鱼的可持续生产和管理水产养殖鱼类的健康，疫苗接种已被发现是增加经济产出和改善动物福利的有效方法。它还允许发展更可持续的养鱼业，减少抗生素和化学品的使用。由于病毒性疾病对全世界水产养殖构成严重威胁，缺乏预防措施会产生广泛的负面后果。例如，胰腺病（PD）是一种由鲑鱼甲病毒（SAV）引起的病毒性鱼类疾病，对挪威鲑鱼水产养殖以及其他欧洲国家（例如挪威）产生了重大影响。英国。另一种鱼类病毒性疾病是病毒性出血性败血症 (VHS)，它是由 VHS 病毒引起的，它会影响淡水或海水中饲养的大多数鲑科鱼类。 VHSV 对养殖虹鳟鱼造成的经济损失是芬兰水产养殖业面临的一项艰巨任务。挪威和英国也爆发了 VHShave。 VHSV已在海水鱼类中检测到，并且已知具有高突变率和适应性。因此，迫切需要开发低成本鱼类疫苗来协助欧洲的可持续水产养殖生产。因此，我们三个欧洲伙伴（挪威、英国和芬兰）的目标是推进现有的植物重组蛋白生产技术，建立跨国植物基低成本疫苗生产平台，以促进未来鱼疫苗的大量低成本生产。我们选择PD和VHS作为原理验证的两个案例，并将测试我们三种不同的烟草生产技术（即通过生产病毒样颗粒的瞬时表达、叶绿体基因工程和烟草BY-2细胞系平台）。通过比较这三个系统的总生产成本、扩大规模潜力和项目期结束时的时间表，PlantVac 项目可以为未来生产 PD 和 VHS 疫苗以及其他鱼类疫苗提供基于技术的植物生产平台。 PlantVac 联盟由经验丰富的科学家组成，他们在拟定的三个植物生产系统、鱼类健康、兽医免疫学和经济学方面拥有所需的专业知识。

项目成果

Synthetic plant virology for nanobiotechnology and nanomedicine.

• DOI: 10.1002/wnan.1447
• 发表时间: 2017-07
• 期刊: Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology
• 作者: Steele JFC;Peyret H;Saunders K;Castells-Graells R;Marsian J;Meshcheriakova Y;Lomonossoff GP
• 通讯作者: Lomonossoff GP

Producing Vaccines against Enveloped Viruses in Plants: Making the Impossible, Difficult.

• DOI: 10.3390/vaccines9070780
• 发表时间: 2021-07-13
• 期刊: Vaccines
• 影响因子: 7.8
• 作者: Peyret H;Steele JFC;Jung JW;Thuenemann EC;Meshcheriakova Y;Lomonossoff GP
• 通讯作者: Lomonossoff GP