ANIHWA call2 - A bacteriophage-based approach to reducing infections caused by antibiotic resistant Escherichia coli

ANIHWA call2 - 一种基于噬菌体的方法，可减少抗生素耐药性大肠杆菌引起的感染

• 批准号: BB/M028399/1
• 负责人: Robert Atterbury
• 金额: $ 45.39万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM028399%2F1
• 关键词: ANIHWA; call2; bacteriophage; based; approach

Antibiotic resistance is a significant and increasing problem in many types of bacteria which cause disease (pathogens) in animals and humans. Escherichia coli is among the most important of these pathogens, because of its role in intestinal, urinary tract and respiratory disease, and septicaemia in a variety of livestock species, including poultry; and also because many strains of E. coli that are associated with septicaemic infections in animals and humans are closely related. Antibiotic resistance in E. coli strains is increasing worldwide and this resistance can be maintained even after reducing or withdrawing antibiotic use. As such, the treatment of E. coli infections in animals and humans requires a new and sustainable approach. This project will investigate the use of bacteriophage as a biological control against harmful strains of E. coli which infect chickens. Bacteriophage, often contracted to 'phage', are viruses which infect and kill bacteria. They are quite specific, only affecting the targeted bacterial species while leaving other bacterial flora unharmed. Phages do not infect animals or humans and are widely distributed in the environment. As such, the use of phages to selectively kill harmful strains of E. coli which infect animals has the potential to be a natural and sustainable alternative to antibiotics, and may also result in new treatments for antibiotic resistant bacterial infections in humans. The effective application of phage therapy will require a thorough understanding of phage-bacteria interactions in a range of environments. This project will use laboratory experiments to build a comprehensive understanding of how phages infect E. coli strains under different environmental conditions. This information will then be used to design protocols for the optimal use of phage therapy to treat infections in animals.

抗生素耐药性是在动物和人类中引起疾病（病原体）的许多类型的细菌中的显著且日益严重的问题。大肠杆菌是这些病原体中最重要的一种，因为它在肠道、泌尿道和呼吸道疾病以及包括家禽在内的各种牲畜物种的败血症中起作用;与动物和人类败血症感染相关的大肠杆菌密切相关。E.大肠杆菌菌株在世界范围内不断增加，即使在减少或停止使用抗生素后，这种耐药性也可以保持。因此，E.动物和人类的大肠杆菌感染需要一种新的和可持续的方法。本项目将研究利用噬菌体作为生物防治有害的大肠杆菌菌株。感染鸡的大肠杆菌。噬菌体，通常简称为“噬菌体”，是感染和杀死细菌的病毒。它们是相当特异的，只影响目标细菌物种，而不伤害其他细菌植物群。噬菌体不感染动物或人类，广泛分布于环境中。因此，使用抗生素选择性地杀死有害的E.感染动物的大肠杆菌有可能成为抗生素的天然和可持续的替代品，也可能导致人类抗生素耐药性细菌感染的新疗法。噬菌体治疗的有效应用将需要彻底了解在一系列环境中的噬菌体-细菌相互作用。本项目将利用实验室实验来建立一个全面的了解如何感染大肠杆菌。大肠杆菌菌株在不同的环境条件下。然后，这些信息将用于设计最佳使用噬菌体疗法治疗动物感染的方案。

项目成果

Biology and Genomics of an Historic Therapeutic Escherichia coli Bacteriophage Collection.

• DOI: 10.3389/fmicb.2017.01652
• 发表时间: 2017
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Baig A;Colom J;Barrow P;Schouler C;Moodley A;Lavigne R;Atterbury R
• 通讯作者: Atterbury R

Sex pilus specific bacteriophage to drive bacterial population towards antibiotic sensitivity

• DOI: 10.1038/s41598-019-48483-9
• 发表时间: 2019-08-30
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Colom, Joan;Batista, Diego;Barrow, Paul
• 通讯作者: Barrow, Paul