ROLE OF DNA REPAIR IN SUSCEPTIBILITY TO SKIN CANCER

DNA 修复在皮肤癌易感性中的作用

• 批准号: 3201225
• 负责人: PAUL T STRICKLAND
• 金额: $ 15.48万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3201225
• 关键词: DNA repair; basal cell carcinoma; basosquamous cell carcinoma; bioassay; biopsy; cancer risk; chloramphenicol acetyltransferase; human subject; immunologic assay /test; lymphocyte; neoplasm /cancer epidemiology; neoplasm /cancer genetics; occupational disease /disorder; phenotype; radiation carcinogenesis; skin neoplasms; solar radiation; sunburn; tissue /cell culture; transfection; ultraviolet radiation

Ultraviolet radiation in sunlight is the major cause of skin cancer in humans. However, individuals differ considerably in their susceptibility to cancer of the skin as well as cancer in other organs. The overall goal of this project is to understand the mechanisms involved in determining individual susceptibility to skin cancer. This goal will be addressed in a previously identified group of Maryland watermen who are at high risk for non-melanoma skin cancer (basal cell carcinoma and squamous cell carcinoma). Accurate estimates of individual lifetime UV exposures have been calculated for each individual from a combination of interview data, working characteristics, and field and laboratory measurements. therefore, the major risk factor for skin cancer, UV exposure, can be easily controlled. Other phenotypic characteristics which influence risk for skin cancer such as skin pigmentation, ease of sunburning (skin type), and hair color, have been recorded for all individuals. This study will examine the role of DNA repair efficiency in susceptibility to non-melanoma skin cancer. DNA repair efficiency of cancer cases and matched controls will be assessed by two DNA repair assays: a lymphocyte- plasmid reactivation assay and an immunoassay for DNA photoproduct repair in lymphocytes and fibroblasts. Other variables such as lifetime UV exposure, sun sensitivity and skin type will be controlled for in the analyses. Association between age at onset of cancer and DNA repair efficiency will also be examined. The results of this study should i) contribute to our overall understanding of the mechanisms that determine susceptibility to skin cancer (and other cancers) in man, ii) aid in the identification of individuals at elevated risk for skin cancer, and iii) be useful in estimating increased risks to the general population resulting from potential increases in solar UV at the earth's surface.

阳光中的紫外线辐射是皮肤癌的主要原因， 人类 然而，个体的易感性差异很大， 皮肤癌和其他器官的癌症。 总目标 这个项目的目的是了解参与决定 个体对皮肤癌的易感性。 这一目标将在 先前确定的一组马里兰州水上运动员， 非黑色素瘤皮肤癌（基底细胞癌和鳞状细胞癌） 癌）。 个人一生紫外线暴露的准确估计 从访谈数据的组合中为每个人计算， 工作特性以及现场和实验室测量。 因此，在本发明中， 皮肤癌的主要危险因素，紫外线暴露，很容易 控制。 影响皮肤风险的其他表型特征 癌症，如皮肤色素沉着，容易晒伤（皮肤类型），和头发 颜色，已记录所有个人。 本研究将探讨DNA修复效率在易感性中的作用 非黑色素瘤皮肤癌 癌症病例的DNA修复效率和 匹配的对照将通过两种DNA修复试验进行评估：淋巴细胞- 质粒再激活试验和DNA光产物修复免疫试验 在淋巴细胞和成纤维细胞中。 其他变量，如寿命UV 暴露，阳光敏感性和皮肤类型将在控制 分析。 癌症发病年龄与DNA修复的关系 效率也将受到审查。 这项研究的结果应该i）有助于我们全面了解 决定皮肤癌易感性的机制（以及其他 癌症），ii）有助于识别处于升高的 皮肤癌的风险，和iii）在估计增加的风险中是有用的， 由于太阳紫外线的潜在增加， 地球的表面。