GENETICS OF ANTIBODIES TO PERIODONTAL PATHOGENS

龋齿和牙周病折磨大多数人在某些时候 在生活中，是所有年龄组牙齿脱落的主要原因。 的 决定对这些疾病的抵抗力或易感性的因素是 未知 这些疾病都是由牙菌斑引起的 微生物，其中殖民牙齿表面和牙龈沟。 的 这些微生物在口腔中定植的能力取决于它们的 附着在牙齿表面或其他细菌上。 特化细胞表面 结构介导细菌对口腔表面的粘附， 针对这些结构的抗体已经显示出抑制 细菌粘附和定植。 一个有吸引力的解释 疾病易感性的差异是遗传控制的变异 在抗体对牙周膜上粘附相关结构的反应中， 病原体 拟议的项目是根据以前的研究， 对SRBC的血清和唾液抗体应答的遗传控制，是一种 血清和唾液抗体反应的遗传控制的详细研究 纯化的蛋白质抗原（T-1），其介导 粘性放线菌菌株T14 V与唾液包被的羟基磷灰石表面的粘附。 实验将在近交系小鼠中进行，并将使用放射免疫测定法 测定IG同种型特异性和对T-1和A.粘性应变 T14V。 将进行菌株调查， 进行基因分析。 血清和唾液抗T-1抗体 亲本品系、F1杂交和回交小鼠的反应将被 分析，以及同类和重组近交系的反应 小鼠 这些数据的分离和连锁分析将证实 假设对T-1血清和唾液抗体应答 基因决定的，并提供洞察这一性质， 控制 不同的、充分表征的抗T-1血清和唾液将被用于检测抗T-1抗体。 在体内吸附抑制测定中进行测试，以将大小和 抗T1应答的同种型，具有抑制 A.粘性菌株T14 V细胞粘附于“内牙表面”。“这些 研究将有望提供对遗传学作用的理解， 确定疾病易感性并确定 保护性抗T-1抗体应答。 这些信息会很有用 在人类免疫方案的发展，以防止或 控制牙周病。

Caries and periodontal diseases afflict most individuals at some time during life and are the major causes of tooth loss in all age groups. The factors which determine resistance or susceptibility to these diseases are unknown. These diseases are caused by dental plaque associated microorganisms, which colonize the tooth surface and gingival sulcus. The ability of these organisms to colonize the oral cavity depends upon their adherence to the tooth surface or other bacteria. Specialized cell surface structures mediate the adherence of bacteria to oral surfaces, and antibodies directed against these structures have been shown to inhibit bacterial adherence and colonization. An attractive explanation for differences in disease susceptibility is genetically controlled variations in the antibody response to adherence associated structures on periodontal pathogens. The proposed project, which is based on previous studies of genetic control of serum and salivary antibody responses to SRBC, is a detailed study of genetic control of serum and salivary antibody responses to a purified protein antigen (T-1) which mediates the adhrerence of Actinomyces viscosus strain T14V to saliva coated hydroxyapetite surfaces. Experiments will be done in inbred mice and a radioimmunoassay will be used to measure Ig isotype specific and responses to T-1 and A. viscosus strain T14V. A strain survey will be done and appropriate parental strains will be selected for genetic analysis. The serum and salivary anti-T-1 antibody responses of the parental strains, F1 hybrid and backcross mice will be analyzed, as well as the responses of congenic and recombinant inbred mice. Segregation and linkage analysis of these data will substantiate the hypothesis that serum and salivary antibody responses to T-1 are genetically determined and provide insight into the nature of this control. Different, well characterized anti-T-1 sera and salivas will be tested in an in vivo adsorption inhibition assay to correlate magnitude and isotype of the anti-T1 response with the ability to inhibit the adhrence of A. viscosus strain T14V cells to an "in intro tooth surface." These studies will hopefully provide an understanding of the role of genetics in determining disease susceptibility and determine the characteristics of protective anti-T-1 antibody responses. This information would be useful in the development of immunization protocols in humans to prevent or control periodontal diseases.