DIABETES AND PERIODONTITIS MICROBIOLOGY AND IMMUNOLOGY

糖尿病和牙周炎微生物学和免疫学

• 批准号: 3222170
• 负责人: JEROME HABER
• 金额: $ 29.1万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-15 至 1991-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3222170
• 关键词: Actinobacillus; Bacteroides gingivalis; Capnocytophaga; Fusobacterium; Streptococcus; Vibrio; adolescence (12-20); dental disorder diagnosis; early diagnosis; human subject; insulin dependent diabetes mellitus; longitudinal human study; oral bacteria; periodontitis; tooth loss; young adult human (21-34)

Periodontal diseases afflict many individuals and are the major cause of tooth loss in adults. Recent evidence from several laboratories has indicated that specific bacterial species are responsible for the initiation and progression of periodontitis. A recent large scale study of patients with insulin dependent diabetes mellitus (IDDM) has shown that the prevalence of periodontitis in young IDDM patients is 6-10 fold higher than that seen in nondiabetic subjects of similar age. Because periodontitis has been traditionally associated with older age groups, IDDM children are not adequately screened for periodontitis. Early diagnosis and treatment of periodontal infections in IDDM patients are important because infections in IDDM patients often increase insulin requirements. Furthermore, treatment for periodontal infections can result in significant post-treatment reductions in insulin requirements. Although they are at high risk, little is known about the microbiological or immunological aspects of periodontitis in IDDM patients. The proposed project is a detailed cross-sectional and longitudinal study of a large IDDM patient population. IDDM patients at the Joslin Diabetes Center, nondiabetic siblings and unrelated nondiabetic subjects from the dental clinic at Tufts University will be compared for the incidence and severity of periodontitis by conventional clinical examination. These groups will also be compared for the microbial species associated with health and disease activity and the serum and salivary antibody responses to these species. IDDM patients with periodontitis will be monitored and subgingival plaque samples from active periodontal lesions will be sampled and cultured by the specific pathogen method. The following species will be isolated and enumerated: Actinobacillus actinomycetemcommitans, Bacteroides, Capnocytophaga and Streptococcal species as well as Camphylobacter consisus, Eikenella corrodens, Fusobacterlum nucleatum and Wolinella recta. Serum and salivary antibody levels of individual isotypes to each microbial species will be measured by enzyme-linked immunoabsorbent assays. The clinical, microbiological and immunological data will be correlated. On the basis of these data, a diagnostic screening test for periodontitis in IDDM patients will be developed and used in clinical trial. These studies will hopefully provide insights into the pathogenesis of periodontitis IDDM patients, which may be relevant to nondiabetic populations. Furthermore, these studies may validate the usefulness of a diagnostic test for periodontitis in a young, high risk population.

牙周病困扰着许多人，是主要的 成年人牙齿脱落的原因。最近来自几个国家的证据 实验室已经表明，特定的细菌种类是 负责牙周炎的发生和发展。一个 近年来对胰岛素依赖型患者的大规模研究 糖尿病(IDDM)的患病率已经表明 年轻IDDM患者的牙周炎比 见于年龄相近的非糖尿病受试者。因为牙周炎 传统上与老年人群有关，IDDM 儿童没有对牙周炎进行充分的筛查。早些时候 胰岛素依赖型糖尿病患者牙周感染的诊断和治疗 患者很重要，因为IDDM患者的感染通常 增加胰岛素需求。此外，治疗 牙周感染可导致严重的治疗后 胰岛素需求量的减少。尽管他们的风险很高， 对于微生物或免疫学方面的知识知之甚少。 IDDM患者牙周炎的发生情况。拟议的项目是一个 一例大型IDDM的详细横断面和纵向研究 病人群体。乔斯林糖尿病中心的IDDM患者， 非糖尿病兄弟姐妹和非糖尿病受试者 塔夫茨大学的牙科诊所将与 常规临床牙周炎的发病率和严重程度 考试。这些组还将与 与健康和疾病活动有关的微生物种类以及 血清和唾液对这些物种的抗体反应。IDDM 将对牙周炎患者进行监测，并在牙龈下进行 活动性牙周病变的菌斑样本将被抽样 并采用特定病原菌培养方法。以下是 将分离和计数的物种：放线杆菌 放线菌、类杆菌、吞噬微孢子菌和 链球菌属和弯曲杆菌属， 腐蚀性艾肯氏杆菌、核梭杆菌和沃里氏杆菌 雷塔。不同亚型人的血清和唾液抗体水平 每一种微生物都将通过酶联法进行测量 免疫吸附分析。临床、微生物学和 免疫学数据将相互关联。在此基础上 IDDM牙周炎诊断性筛查试验DATA 患者将被开发并用于临床试验。这些研究 将有望为进一步深入了解该病的发病机制提供帮助 牙周炎IDDM患者，这可能与 非糖尿病人群。此外，这些研究可能会验证 牙周炎诊断测试在一名年轻、 高危人群。