EFFECT OF PAROTID FUNCTION ON SALIVA AND CELLS

腮腺功能对唾液和细胞的影响

• 批准号: 3219724
• 负责人: DORTHEA JOHNSON
• 金额: $ 9.84万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-06-01 至 1989-09-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219724
• 关键词: DNA; RNA; animal food; dietary constituent; drug administration rate /duration; gel electrophoresis; ion exchange chromatography; isoproterenol; mastication; nutrition related tag; parotid gland; proline; ribosomes; saliva; salivation; secretion; thymidine

Proline-rich proteins contribute about 70% of the proteins in human parotid saliva. Observations in this laboratory, coupled with those from other laboratories, suggest a stong negative correlation between basic proline-rich proteins in the parotid saliva of rats and caries susceptibility. Very little is known about factors which influence the synthesis of these unique salivary proteins. The long-term objectives of this project have been to study factors which contribute to their regulation. Observations to date in this laboratory, coupled with the findings in other laboratories, suggest that alterations in b-adrenergic receptors lead to parallel changes in the proportion of proline-rich proteins in rat parotid saliva. Thus, the present proposal was designed to study this apparent relationship. The two specific aims are (I) to test the hypothesis that changes in the proportion of proline-rich proteins in rat parotid saliva are associated with parallel disproportionate changes in b-receptor density or biologic activity and (II) to determine at what stage of postnatal development of the rat parotid gland proline-rich proteins appear. A delay in their appearance until b-receptors exhibit biologic activity would support the hypothesis. In these studies the proportion of proline-rich proteins in parotid saliva will be correlated with b-receptor density or biologic activity. The composition of proteins will be determined by densitometric scanning of color positives taken of acrylamide gels following SDS-PAGE. These findings will be correlated with studies which test the biologic activity of the b-receptors as well as their density. The former involves in vitro assays of the ability of receptors to respond to an appropriate stimulus; the latter involves radioligand binding studies for quantitation of numbers of receptors. The proposed work may result in a better understanding of factors which regulate this unique group of salivary proteins.

富含脯氨酸的蛋白质约占人类腮腺蛋白质的 70% 唾液。 本实验室的观察结果与其他实验室的观察结果相结合 实验室表明，基本之间存在很强的负相关性 大鼠腮腺唾液中富含脯氨酸的蛋白质与龋齿 易感性。 对于影响其影响的因素知之甚少 这些独特的唾液蛋白的合成。 的长期目标 这个项目的目的是研究影响他们的因素 规定。 该实验室迄今为止的观察结果，加上 其他实验室的研究结果表明，β-肾上腺素能的改变 受体导致富含脯氨酸的比例发生平行变化 大鼠腮腺唾液中的蛋白质。 因此，本提案旨在 研究这种明显的关系。 两个具体目标是（I）测试 富含脯氨酸的蛋白质的比例发生变化的假设 大鼠腮腺唾液与平行不成比例的变化有关 b-受体密度或生物活性以及 (II) 确定处于哪个阶段 大鼠腮腺富含脯氨酸蛋白的产后发育 出现。 延迟出现，直至 b 受体表现出生物活性 活动将支持该假设。 在这些研究中所占的比例 腮腺唾液中富含脯氨酸的蛋白质将与 b 受体相关 密度或生物活性。 蛋白质的组成将是 通过丙烯酰胺彩色正片的光密度扫描确定 SDS-PAGE 后的凝胶。 这些发现将与研究相关 测试 b 受体及其生物活性 密度。 前者涉及受体能力的体外测定 对适当的刺激做出反应；后者涉及放射性配体 用于定量受体数量的结合研究。 拟议的 工作可能会导致更好地理解调节这一点的因素 一组独特的唾液蛋白。