EFFECT OF PAROTID FUNCTION ON SALIVA & CELLS

腮腺功能对唾液的影响

• 批准号: 3219725
• 负责人: DORTHEA JOHNSON
• 金额: $ 12.49万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-06-01 至 1993-09-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219725
• 关键词: DNA; RNA biosynthesis; adrenergic agents; autoradiography; beta adrenergic receptor; dietary constituent; drug administration rate /duration; electrophoresis; glucocorticoids; hormone regulation /control mechanism; laboratory rat; mastication; messenger RNA; neurotransmitters; nutrition related tag; oral bacteria; parasympathetic nervous system; parotid gland; protein biosynthesis; saliva; salivation; secretion; sympathetic nervous system; thyroid hormones

Proline-rich proteins contribute about 70% of the proteins in human parotid saliva. Observations in this laboratory, coupled with those from other laboratories, suggest a stong negative correlation between basic proline-rich proteins in the parotid saliva of rats and caries susceptibility. Very little is known about factors which influence the synthesis of these unique salivary proteins. The long-term objectives of this project have been to study factors which contribute to their regulation. Observations to date in this laboratory, coupled with the findings in other laboratories, suggest that alterations in b-adrenergic receptors lead to parallel changes in the proportion of proline-rich proteins in rat parotid saliva. Thus, the present proposal was designed to study this apparent relationship. The two specific aims are (I) to test the hypothesis that changes in the proportion of proline-rich proteins in rat parotid saliva are associated with parallel disproportionate changes in b-receptor density or biologic activity and (II) to determine at what stage of postnatal development of the rat parotid gland proline-rich proteins appear. A delay in their appearance until b-receptors exhibit biologic activity would support the hypothesis. In these studies the proportion of proline-rich proteins in parotid saliva will be correlated with b-receptor density or biologic activity. The composition of proteins will be determined by densitometric scanning of color positives taken of acrylamide gels following SDS-PAGE. These findings will be correlated with studies which test the biologic activity of the b-receptors as well as their density. The former involves in vitro assays of the ability of receptors to respond to an appropriate stimulus; the latter involves radioligand binding studies for quantitation of numbers of receptors. The proposed work may result in a better understanding of factors which regulate this unique group of salivary proteins.

富含脯氨酸的蛋白质约占人腮腺蛋白质的70% 唾液。本实验室的观察结果，再加上其他实验室的观察结果 实验室，表明基本的 大鼠和龋病大鼠腮腺唾液中富含脯氨酸的蛋白质 敏感度。人们对影响生物多样性的因素知之甚少。 这些独特的唾液蛋白质的合成。的长期目标 这个项目一直在研究影响他们的因素 监管。到目前为止在这个实验室的观察结果，再加上 其他实验室的研究结果表明，b型肾上腺素能 受体导致富含脯氨酸的比例发生平行变化 大鼠腮腺唾液中的蛋白质。因此，本提案旨在 研究这种明显的关系。这两个具体目标是：(一)测试 一种假说是富含脯氨酸的蛋白质比例的变化 大鼠腮腺唾液与平行的不成比例的变化有关 B受体密度或生物活性以及(Ii)确定在什么阶段 大鼠腮腺富含脯氨酸蛋白的出生后发育 出现。它们出现的延迟，直到b受体表现出生物学特性 经济活动将支持这一假设。在这些研究中， 腮腺唾液中富含脯氨酸的蛋白质将与b受体相关 密度或生物活性。蛋白质的组成将是 通过对丙烯酰胺的彩色正片进行密度扫描确定 凝胶跟随十二烷基硫酸钠-PAGE。这些发现将与研究相关联 它们测试了b受体的生物活性以及它们的 密度。前者涉及到受体能力的体外分析。 对适当的刺激作出反应；后者涉及放射性配基 受体数量定量的结合研究。建议数 工作可能会使我们更好地理解调节这一现象的因素。 一组独特的唾液蛋白质。