ERASynBio2-SMARTPLANTS

ERASynBio2-智能植物

• 批准号: BB/N010248/1
• 负责人: Philip Wigge
• 金额: $ 61.83万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN010248%2F1
• 关键词: ERASynBio2; SMARTPLANTS

A major goal of plant synthetic biology is to create smart plants that are able to respond to key cues and display a variety of agronomically valuable traits such as enhanced stress resilience or the biosynthesis of high value compounds. The objectives of the SMARTPLANTS consortium are to develop parallel regulatory networks (PaRNets) that are based on cues that plants normally encounter in their growth cycle, namely flowering and temperature changes, and translate these into metabolic engineering-based outputs to produce high value or stress-protecting compounds. Flowering is accompanied by a dramatic metabolic switch leading to the massive transfer of resources from the leaves to the seeds or the fruits. However there is still significant biomass remaining in the leaves and stems. By developing a PaRNet that uses flowering as a trigger, we will capture part of this biomass to convert it to a high value compound, the diterpene cis-abienol. Our flowering PaRNet will be based on the florigen signal encoded by the conserved FT gene. Similarly we will develop a PaRNet based on temperature fluctuations to induce upon higher temperatures the production of isoprene, a compound conferring heat-stress protection. One key aspect in the design of these networks is signal propagation, which will be mediated by mobile orthogonal Transcription Activator-Like Effectors (TALEs) activating synthetic promoters. Mobility will be engineered by fusing the TALEs to Intercellular Trafficking Motifs or viral movement proteins, or by using deconstructed viral vectors. Both regulatory network and metabolic engineering optimisation will be assisted by modelling in iterative rounds. Finally, to initiate and promote a community effort in the development of artificial plant regulatory networks we will organize an international symposium on this topic. SMARTPLANTS is a true interdisciplinary project, which will be carried out by a team of highly experienced scientists with complementary skills

植物合成生物学的一个主要目标是创造智能植物，这些植物能够对关键线索做出反应，并显示出各种具有农学价值的性状，例如增强的抗逆性或高价值化合物的生物合成。SMARTPLANTS联盟的目标是开发基于植物在其生长周期中通常遇到的线索（即开花和温度变化）的平行调控网络（PaRNets），并将其转化为基于代谢工程的输出，以产生高价值或应力保护化合物。开花伴随着一个戏剧性的代谢转换，导致大量的资源从叶子转移到种子或果实。然而，在叶和茎中仍然存在显著的生物量。通过开发一种以开花为触发器的PaRNet，我们将捕获部分生物量，将其转化为高价值的化合物，即二萜顺式冷杉醇。我们的开花PaRNet将基于由保守的FT基因编码的成花素信号。同样，我们将开发一种基于温度波动的PaRNet，以在较高温度下诱导异戊二烯的产生，异戊二烯是一种提供热应力保护的化合物。设计这些网络的一个关键方面是信号传播，其将由激活合成启动子的移动的正交转录激活因子样效应子（TALE）介导。将通过将TALE与细胞间运输基序或病毒移动蛋白融合或通过使用解构的病毒载体来工程化移动性。调控网络和代谢工程优化都将通过迭代轮中的建模来辅助。最后，为了发起和促进人工植物调控网络发展的社区努力，我们将组织一次关于这一主题的国际研讨会。智能植物是一个真正的跨学科项目，将由一组经验丰富的科学家进行，他们具有互补的技能。

项目成果

Transcriptional Regulation of the Ambient Temperature Response by H2A.Z Nucleosomes and HSF1 Transcription Factors in Arabidopsis.

• DOI: 10.1016/j.molp.2017.08.014
• 发表时间: 2017-10-09
• 期刊: Molecular plant
• 影响因子: 27.5
• 作者: Cortijo S;Charoensawan V;Brestovitsky A;Buning R;Ravarani C;Rhodes D;van Noort J;Jaeger KE;Wigge PA
• 通讯作者: Wigge PA

Phytochromes function as thermosensors in Arabidopsis

• DOI: 10.1126/science.aaf6005
• 发表时间: 2016-11-18
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Jung, Jae-Hoon;Domijan, Mirela;Wigge, Philip A.
• 通讯作者: Wigge, Philip A.

The evening complex coordinates environmental and endogenous signals in Arabidopsis.

• DOI: 10.1038/nplants.2017.87
• 发表时间: 2017-06-26
• 期刊: Nature plants
• 影响因子: 18
• 作者: Ezer D;Jung JH;Lan H;Biswas S;Gregoire L;Box MS;Charoensawan V;Cortijo S;Lai X;Stöckle D;Zubieta C;Jaeger KE;Wigge PA
• 通讯作者: Wigge PA

Phytochromes function as thermosensors in $\textit{Arabidopsis}$

光敏色素在$ extit{拟南芥}$中充当热传感器

• DOI: 10.17863/cam.7172
• 发表时间: 2016
• 作者: Jung J