FUNTIONAL MORPHOLOGY OF THE OLFACTORY BULB

嗅球的功能形态

• 批准号: 3217777
• 负责人: PASQUALE P GRAZIADEI
• 金额: $ 4.23万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3217777
• 关键词: aspartate; cell type; cholecystokinin; corticotropin releasing factor; electron microscopy; endorphins; gamma aminobutyrate; glutamates; glycine; immunocytochemistry; morphology; neurochemistry; neurons; olfactory lobe; serotonin; somatostatin; substance P; synapses

The putative role of the vomeronasal chemosensory system is to receive species-specific odors such as pheromones. In fact, its central connections project to brain structures involved in reproduction. However, so far, little is known about its information processing mechanisms. The long term goal of this project is to contribute to our understanding of the morphological pattern of the accessory olfactory bulb to better clarify its working mechanisms. Our program intends to characterize the types of neurons of the accessory olfactory bulb by describing their morphology, synaptology and immunocytochemical characteristics, and to examine if there are some anatomical and/or chemical correction(s) between these neurons and the secondary brain centers of the vomeronasal system. To achieve this goal, the morphology and the synaptology of the neurons of the accessory olfactory bulb will be studied by light- and electron-microscopic procedures. Specific relevant antigens will be localized with appropriate immunocytochemical methods. Direct correlation between the morphology and the chemical characteristics will be achieved by double staining of the same neurons by immunocytochemical and Golgi procedures. Furthermore, the tracing methods will be used to determine the projection areas of output neurons of the accessory olfactory bulb. This project will help in our understanding of the olfactory sensory mechanisms and consequently disorders of the olfactory system.

犁鼻化学感觉系统的假定作用是接受 物种特有的气味，如信息素。事实上，它的中心 连接投射到与生殖有关的大脑结构。 然而，到目前为止，人们对它的信息处理知之甚少 机制。这个项目的长期目标是为我们的 对副嗅觉形态结构的认识 以更好地阐明其工作机制。我们的节目打算 通过观察副嗅球神经元的类型 描述它们的形态、突触和免疫细胞化学 特征，并检查是否有一些解剖学和/或 这些神经元与次级脑之间的化学校正(S) 犁鼻系统的中心。 为了实现这一目标，神经元的形态和突触 将通过光和光来研究副嗅球的 电子显微镜程序。特定的相关抗原将是 用适当的免疫细胞化学方法进行定位。直接 形态和化学特征之间的关联将会 通过免疫细胞化学对相同的神经元进行双重染色来实现 和高尔基手术。此外，还将使用跟踪方法来 确定副神经输出神经元的投射面积 嗅球。这个项目将有助于我们理解 嗅觉的感觉机制以及由此导致的嗅觉障碍 系统。