BRAIN TRANSPLANTATION AND OLFACTORY NEURONS INTERACTION

脑移植和嗅觉神经元相互作用

• 批准号: 3401244
• 负责人: PASQUALE P GRAZIADEI
• 金额: $ 13.5万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3401244
• 关键词: autoradiography; axon; central nervous system; cerebellar cortex; electron microscopy; histochemistry /cytochemistry; histology; immunochemistry; mature animal; medial olfactory area; nervous system transplantation; neural plasticity; neuroanatomy; newborn animals; olfactory lobe; parietal lobe /cortex; tritium; visual cortex

In this program we propose to study the role that the olfactory bulb plays in the development, maturation and survival of the olfactory sensory neurons. We intend also to study the role that the olfactory sensory neurons play in the development of the olfactory bulb target neurons (mitral and tufted cells). In the first part of the study we will test the behavior of the olfactory neurons in adult rats which were partially or totally deprived of the olfactory bulb as neonatals or adults. We will also, by means of transplantation, substitute the olfactory bulb with the presumptive visual cortex (a non olfactory target) from E14 embryos. The changes in the development, maturation and survival that we expect to be expressed by an altered turnover rate, will be assessed by means of quantitative morphology, 3H Thymidine autoradiography and immunohistochemistry for the demonstration of the olfactory marker protein. These data will be compared with the results obtained in normal animals. In the second part of the program (role of the olfactory sensory neurons in the development of their target (the olfactory bulb)), we will transplant the presumptive olfactory bulb area from E14 rat embryos in place of the olfactory bulb of a neonatal rat (P1) after its olfactory bulb has been totally removed. Furthermore, we will also transplant the same presumptive olfactory bulb area into the parietal cortex of neonatal rats and into the anterior chamber of the eye of adult rats to see their development without the influence of the olfactory input. As a control, the development of the normal olfactory bulb will be studied in stage embryos and postnatal rats. Silver methods, TEM and immunohistochemical techniques will be employed to evaluate the development of the large neurons of the olfactory bulb.

在这个程序中，我们建议研究的作用，嗅觉 鳞茎在植物的发育、成熟和生存中起着重要的作用。 嗅觉神经元 我们还打算研究 嗅觉感觉神经元在嗅觉神经元的发育过程中起着重要作用。 嗅球靶向神经元（僧帽细胞和簇状细胞）。 在研究的第一部分中，我们将测试 成年大鼠的嗅觉神经元，部分或全部 在幼年或成年时失去了嗅球。 我们将 并通过移植的方法，替代嗅球 与假定的视觉皮层（非嗅觉目标）， E14胚胎。 在发展、成熟和 我们期望通过改变营业额来表达的生存 率，将通过定量形态学方法进行评估，3H 胸腺嘧啶核苷放射自显影和免疫组化 嗅觉标记蛋白的演示。 这些数据将 与在正常动物中获得的结果进行比较。 在节目的第二部分（嗅觉的作用 神经元在其目标（嗅球）的发育中）， 我们将从E14移植假定的嗅球区域 大鼠胚胎代替新生大鼠的嗅球（P1） 在它的嗅球被完全切除后 此外，委员会认为， 我们也会移植同样的嗅球区域 进入新生大鼠的顶叶皮层， 成年大鼠的眼睛的腔室，看看他们的发展，没有 嗅觉输入的影响 作为对照， 正常嗅球的发育将分阶段进行研究 胚胎和出生后的大鼠。 银法、透射电镜和 免疫组织化学技术将被用来评估 嗅球大神经元的发育。