BRAIN TRANSPLANTATION AND OLFACTORY NEURONS INTERACTION

我们建议研究嗅觉感觉神经元之间的相互作用 以及嗅球和小脑的神经元群 皮质，通过移植技术。 两个皮质将 移植到靠近筛板的颅内侧， 研究它们与嗅觉感觉轴突的相互作用 神经元留在原位。 随后，嗅觉神经元（包含 在嗅粘膜碎片中），将被直接移植到 新生和成年大鼠的嗅球和小脑，研究它们的 当这些皮层留在原位时与这些皮层相互作用。 在 第三个实验嗅觉神经元和皮层都将被 移植到眼前房以研究它们的相互作用 当两个组织都从正常位置移除时。 初步研究表明，当嗅觉神经元 被移植远离鼻腔，它们迁移远离鼻腔 神经上皮并进入宿主组织。 命运与可能 迁移的嗅觉神经元与宿主的中枢神经系统的相互作用会 被调查。 拟议的研究将使用组织学技术以及 超微结构、放射自显影和免疫组织化学方法。 的 计划建议定义嗅觉神经元的感应能力 在各种实验环境中并研究它们的可塑性 和迁移潜力。

We propose to study the interaction between the olfactory sensory neurons and the neuronal populations of the olfactory bulbar and cerebellar cortexes, by means of transplantation techniques. The two cortexes will be transplanted close to the intracranial side of the lamina cribrosa, to study their interaction with the olfactory sensory axons when the olfactory neurons are left in situ. Subsequently, the olfactory neurons (contained in fragments of olfactory mucosa), will be transplanted directly into the olfactory bulb and cerebellum of neonatal and adult rats to study their interaction with these cortexes when the latter are left in situ. In the third experiment both the olfactory neurons and the cortexes will be transplanted in the anterior chamber of the eye to study their interaction when both tissues are removed from their normal locations. Prelimininary studies have demonstrated that, when the olfactory neurons are transplanted away from the nasal cavity, they migrate away from the neuroepithelium and into the host's tissue. The destiny and the possible innteraction of the migrating olfactory neurons with the host's C.N.S. will be investigated. The proposed study will use histological techniques as well as ultrastructural, autoradiographic and immunohistochemical methods. The program proposes to define the inductive capacity of the olfactory neurons in a variety of experimental setting and to investigate their plasticity and migratory potential.