BRAIN TRANSPLANTATION AND OLFACTORY NEURONS INTERACTION

我们建议研究嗅觉感觉神经元之间的相互作用 以及嗅球和小脑的神经元群 皮质，通过移植技术。 这两个皮层 移植到靠近筛板的颅内侧， 研究它们与嗅觉感觉轴突的相互作用， 神经元留在原位。 随后，嗅觉神经元（包含 在嗅粘膜碎片中），将直接移植到 嗅球和小脑，以研究其对新生和成年大鼠的 与这些皮质的相互作用，当后者留在原位。 在 第三个实验嗅觉神经元和皮层都将被 植入眼前房，研究它们之间的相互作用 当两个组织都从它们的正常位置被移除时。 初步研究表明，当嗅觉神经元 从鼻腔中移走， 神经上皮细胞进入宿主组织 命运与可能 迁移的嗅觉神经元与宿主中枢神经系统的相互作用。将 追究 拟议的研究将使用组织学技术以及 超微结构、放射自显影和免疫组织化学方法。 的 该计划建议定义嗅觉神经元的感应能力， 并研究其可塑性 和迁移潜力。

We propose to study the interaction between the olfactory sensory neurons and the neuronal populations of the olfactory bulbar and cerebellar cortexes, by means of transplantation techniques. The two cortexes will be transplanted close to the intracranial side of the lamina cribrosa, to study their interaction with the olfactory sensory axons when the olfactory neurons are left in situ. Subsequently, the olfactory neurons (contained in fragments of olfactory mucosa), will be transplanted directly into the olfactory bulb and cerebellum of neonatal and adult rats to study their interaction with these cortexes when the latter are left in situ. In the third experiment both the olfactory neurons and the cortexes will be transplanted in the anterior chamber of the eye to study their interaction when both tissues are removed from their normal locations. Prelimininary studies have demonstrated that, when the olfactory neurons are transplanted away from the nasal cavity, they migrate away from the neuroepithelium and into the host's tissue. The destiny and the possible innteraction of the migrating olfactory neurons with the host's C.N.S. will be investigated. The proposed study will use histological techniques as well as ultrastructural, autoradiographic and immunohistochemical methods. The program proposes to define the inductive capacity of the olfactory neurons in a variety of experimental setting and to investigate their plasticity and migratory potential.