Optimising ammonia to improve sustainability in highly buffered recirculating aquaculture systems (RAS)

优化氨以提高高缓冲循环水产养殖系统 (RAS) 的可持续性

• 批准号: BB/N013344/1
• 负责人: Rod Wilson
• 金额: $ 19.15万
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN013344%2F1
• 关键词: Optimising; ammonia; improve; sustainability; highly

The current FLIP proposal builds upon an existing BBSRC Industrial Partnership Award in collaboration with Skretting (the largest global producer of aquaculture feed). The latter IPA project is based on a novel manipulation of diets that has already been demonstrated to improve the efficiency of converting food into growth by a remarkable 20 % under laboratory conditions. It uses laboratory studies with live fish to assess the energetic costs and health implications of feeding in aquaculture fish, and then to design optimal diet compositions to minimise these costs. It aims to make energetic savings for the fish in particular regarding acid-base and salt balance following a meal, and minimise how these natural disturbances impact upon respiratory gas exchange and excretory processes. The present FLIP proposal will use similar approaches to the above current BBSRC-funded project. However, whereas the current BBSRC-IPA project addresses dietary issues, this FLIP proposal specifically addresses newly discovered water quality changes that are particularly associated with intensive recirculating aquaculture systems (RAS). The present FLIP proposal is also linked to another FLIP project (started June 2015) as both stem from water quality issues experienced by intensive RAS. Specifically the artificially enhanced buffer capacity of the water (raised NaHCO3 as part of the regulation of water pH). In turn this inhibits ammonia excretion in fish (through buffering of the normally acidic gill boundary layer), causing increases in blood ammonia which can result in undesirable changes in whole animal performance. The present FLIP proposal seeks to use a 2-way interchange between academia and industry to address previously unconsidered factors that can have a major influence on the biology and efficiency of growth in fish. By facilitating an interchange of academic and industrial personnel between their respective sites this project aims to address these non-ideal changes in water chemistry associated with intensive recirculating aquaculture systems (RAS). It aims to establish (and ideally prevent) previously unrecognised energetic costs for fish associated with regulation of internal ammonia levels caused by these water quality issues that can impair health, welfare, growth and ultimately the production efficiency in aquaculture. It is a collaboration with Anglesey Aquaculture Ltd (AAL), the largest marine RAS in Europe and the UK's only farm for seabass, a high value and commercially important fish. This form of land-based aquaculture is increasingly promoted worldwide due to its sustainability in terms of low water use and minimising environmental problems from waste products. However, the intensity of the aquaculture conditions creates water quality problems that must be countered, primarily a consumption of oxygen and excretion of carbon dioxide by the fish that acidifies the water. To deal with this pH problem RAS operators add huge amounts of alkali (hydroxide or bicarbonate). However, this pH compensation measure creates a further water quality issue, specifically high alkalinity (and therefore buffer capacity), whilst CO2 is only partially reduced. These secondary changes are known to inhibit the excretion of ammonia by fish gills, raising internal blood levels of ammonia, and ultimately impair the general physiology and energetics of fish, and negatively impacting their feeding and growth. By facilitating a 2-way transfer of knowledge and skills (via direct secondments of one academic and one industry interchanger, at each other's site), this FLIP project aims to provide a cost-effective, evidence-based solution(s) to these specific water quality issues. Furthermore, we aim to embed a culture of problem-solving through academic-industrial collaboration into the fabric of both organisations such that future problems associated with sustainable production of fish can be avoided or mitigated in a timely fashion.

目前的FLIP提案建立在与Skretting（全球最大的水产养殖饲料生产商）合作的现有BBSRC工业伙伴关系奖的基础上。后一个IPA项目基于一种新颖的饮食操作，已被证明可以在实验室条件下将食物转化为生长的效率显着提高20%。它使用活鱼的实验室研究来评估养殖鱼类的能量成本和健康影响，然后设计最佳的饮食组合以最大限度地减少这些成本。它旨在为鱼类节省能量，特别是在餐后的酸碱和盐平衡方面，并尽量减少这些自然干扰对呼吸气体交换和排泄过程的影响。目前的FLIP提案将使用与上述BBSRC资助的项目类似的方法。然而，尽管目前的BBSRC-IPA项目解决了饮食问题，但FLIP提案专门解决了新发现的水质变化，这些变化特别与密集循环水产养殖系统（RAS）有关。目前的FLIP提案还与另一个FLIP项目（2015年6月开始）有关，因为两者都源于密集RAS遇到的水质问题。特别是人为增强水的缓冲能力（提高NaHCO 3作为调节水pH值的一部分）。反过来，这又抑制了鱼的氨排泄（通过缓冲正常的酸性鳃边界层），导致血氨增加，这可能导致整个动物性能的不良变化。目前的FLIP提案旨在利用学术界和工业界之间的双向交流来解决以前未考虑的因素，这些因素可能对鱼类的生物学和生长效率产生重大影响。通过促进学术和工业人员在各自地点之间的交流，该项目旨在解决与密集循环水产养殖系统（RAS）相关的水化学的这些非理想变化。它旨在确定（并理想地防止）与这些水质问题引起的内部氨水平调节相关的鱼类先前未被认可的能量成本，这些水质问题可能会损害健康，福利，生长并最终影响水产养殖的生产效率。它是与Anglesey Aquarium Ltd（AAL）合作的，Anglesey Aquarium Ltd是欧洲最大的海洋RAS，也是英国唯一的鲈鱼养殖场，这是一种高价值和商业重要的鱼类。这种形式的陆地水产养殖由于其在低用水量和最大限度地减少废物产生的环境问题方面的可持续性而在全球范围内得到越来越多的推广。然而，水产养殖条件的强度产生了必须解决的水质问题，主要是鱼类消耗氧气和排泄二氧化碳，使水酸化。为了解决这个pH值问题，RAS操作员添加了大量的碱（氢氧化物或碳酸氢盐）。然而，这种pH补偿措施产生了进一步的水质问题，特别是高碱度（以及因此的缓冲能力），而CO2仅部分减少。已知这些次级变化抑制鱼鳃排泄氨，提高内部血液氨水平，并最终损害鱼的一般生理和能量学，并对其摄食和生长产生负面影响。通过促进知识和技能的双向转移（通过直接借调一个学术和一个行业交换者，在对方的网站），该FLIP项目旨在为这些特定的水质问题提供一个具有成本效益的，基于证据的解决方案。此外，我们的目标是通过学术-工业合作将解决问题的文化嵌入到两个组织的结构中，以便及时避免或减轻与可持续鱼类生产相关的未来问题。

项目成果

Supplementary Figures from Molecular and biochemical characterization of the bicarbonate-sensing soluble adenylyl cyclase from a bony fish, the rainbow trout

来自虹鳟鱼的碳酸氢盐感应可溶性腺苷酸环化酶的分子和生化特征的补充数据

• DOI: 10.6084/m9.figshare.13537192
• 发表时间: 2021
• 作者: Salmerón C

Supplementary Methods from Molecular and biochemical characterization of the bicarbonate-sensing soluble adenylyl cyclase from a bony fish, the rainbow trout

虹鳟鱼碳酸氢盐感应可溶性腺苷酸环化酶的分子和生化表征的补充方法

• DOI: 10.6084/m9.figshare.13537183
• 发表时间: 2021
• 作者: Salmerón C

Molecular and biochemical characterization of the bicarbonate-sensing soluble adenylyl cyclase from a bony fish, the rainbow trout Oncorhynchus mykiss.

来自硬骨鱼虹鳟鱼 Oncorhynchus mykiss 的碳酸氢盐感应可溶性腺苷酸环化酶的分子和生化特征。

• DOI: 10.1098/rsfs.2020.0026
• 发表时间: 2021
• 期刊: Interface focus
• 影响因子: 4.4
• 作者: Salmerón C

Table S2 from Molecular and biochemical characterization of the bicarbonate-sensing soluble adenylyl cyclase from a bony fish, the rainbow trout

表 S2 来自硬骨鱼虹鳟的碳酸氢盐感应可溶性腺苷酸环化酶的分子和生化特征

• DOI: 10.6084/m9.figshare.13537186
• 发表时间: 2021
• 作者: Salmerón C

Supplementary material - Systematic Map Protocol from Does sex really matter? Explaining intraspecies variation in ocean acidification responses

补充材料 - 系统地图协议来自性别真的很重要吗？

• DOI: 10.6084/m9.figshare.4556008
• 发表时间: 2017
• 作者: Ellis R