OTITIS MEDIA WITH EFFUSION: HUMAN STUDIES

渗出液的中耳炎：人类研究

• 批准号: 3215638
• 负责人: THOMAS F DEMARIA
• 金额: $ 53.97万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-09-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3215638
• 关键词: antibiotics; bacterial cytopathogenic effect; bactericidal immunity; chinchilla; cholesteatoma; complement; disease /disorder model; disease /disorder prevention /control; ear disorder diagnosis; electron microscopy; histochemistry /cytochemistry; human tissue; immune tolerance /unresponsiveness; immunochemistry; immunoglobulins; immunopathology diagnosis; laboratory mouse; laboratory rabbit; lysozyme; microorganism classification; microorganism culture; microorganism immunology; model design /development; otitis media; phagocytosis; preschool child (1-5); protease inhibitor; respiratory infections; virus cytopathogenic effect

Otitis media with effusion (OME), or serous otitis media, is a common condition in children and frequently leads to a moderate deafness at a critical age of development. The long-term objectives of this study are: 1) to determine the pathogenesis of OME in humans, 2) to develop suitable animal models for OME, and 3) to develop a scientific rationale for diagnostic, preventive and management strategies for OME. The specific objectives are to define the roles of tubal dysfunction, antecedent acute bacterial otitis media, respiratory virus, bacterial (or viral) antigens, bacteria- (or virus-) host interaction, immune protection, immune injury, and antibiotics in the pathogenesis of OME, using microbiological, immunochemical, histopathological and immunocytochemical techniques in human OME patients and in animal experimental models. The major hypotheses to test are: 1. Initial infection may be modified by the local production of bacteriostatic substances (immunoglobulins and lysozyme), impaired phagocytic function, and/or inadequate antibiotic treatment, leading to OME. 2. After initial infection, dead bacteria or bacterial (or viral) antigens may remain in the middle ear due to poor tubal function, which can elicit immune injury or nonspecific activation of mediators of inflammation, leading to OME. Such substances include endotoxin and peptidoglycan. 3. Viral infection (upper respiratory tract) may predispose the middle ear to bacterial infection due to suppression of immune responses and/or impairment of local clearance mechanisms. 4. Early antibiotic treatment of an initial ear infection prevents the development of adequate immunity of the ear, particularly in the young age group (under two years of age) whose immune systems are being developed, and subsequent infection results in OME due to the incomplete development of immunity in the middle ear.

带有积液（OME）或浆液性中耳炎的中耳炎是常见的 儿童的状况，经常导致中度的耳聋 关键的发展年龄。 这项研究的长期目标是：1）确定 人类中ome的发病机理，2）开发合适的动物模型 OME和3）为诊断，预防性开发科学原理 和OME的管理策略。 具体目标是定义 输卵管功能障碍，先前急性细菌性中炎培养基的作用， 呼吸道病毒，细菌（或病毒）抗原，细菌 - （或病毒） 宿主相互作用，免疫保护，免疫损伤和抗生素 使用微生物，免疫化学的发病机理， 人OME患者的组织病理学和免疫细胞化学技术 和动物实验模型。 要测试的主要假设是： 1。最初的感染可以通过局部生产来改变 噬抑制物质（免疫球蛋白和溶菌酶）受损 吞噬功能和/或抗生素治疗不足，导致OME。 2。初次感染后，死细菌或细菌（或病毒）抗原 由于输卵管功能不良，可能会留在中耳中 免疫损伤或非特异性炎症介体激活， 导致ome。 这种物质包括内毒素和肽聚糖。 3。病毒感染（上呼吸道）可能会易于中耳 由于抑制免疫反应和/或 局部清除机制的损害。 4。初始耳朵感染的早期抗生素治疗可防止 尤其是在年轻时的足够免疫力的发展 正在开发免疫系统的组（两岁以下）， 随后由于发展不完整而导致OME导致OME 中耳中的免疫力。