FIBRINOGEN BINDING AND VIRULENCE OF BACTEROIDES SPECIES

拟杆菌属的纤维蛋白原结合和毒力

• 批准号: 3220874
• 负责人: MARILYN S LANTZ
• 金额: $ 20.06万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-09-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3220874
• 关键词: Bacteroides; Macaca; binding proteins; fibrinogen; fibronectins; gingivitis; laboratory mouse; laboratory rabbit; oral bacteria; periodontitis; virulence

Some forms of gingivitis and periodontitis are associated with proportional increases (emergence) of indigenous black-pigmented Bacteroides species in the subgingival microbiota, particularly Bacteroides gingivalis and Bacteroides intermedius. It is not known what virulence factor or factors permit emergence of these organisms, or whether emergence of these organisms contributes to the pathogenesis of gingivitis and periodontitis. A number of B. gingivalis and B. intermedius strains bind and adhere to fibrinogen and fibrin in vitro. Binding and adherence of these strains to fibrinogen are mediated by a bacterial protein (Mr 200k). Studies are proposed to investigate whether fibrinogen binding protein (FBP) is an in vivo virulence factor necessary for emergence of B. gingivalis in developing periodontitis lesions in the cynomolgus monkey. Elucidation of mechanisms of emergence of Bacteroides species is important in understanding their etiologic role in periodontal diseases. An understanding of these mechanisms is also necessary to achieve the long range goal of this research which is to develop strategies to block emergence of these organisms and thereby block the development of periodontitis. The following Specific Aims are proposed: 1. To purify fibrinogen binding proteins from human and cynomolgus strains of Bacteroides gingivalis and Bacteroides intermedius. 2. To characterize and compare fibrinogen binding proteins from monkey and human strains of Bacteroides gingivalis and Bacteroides intermedius. 3. To determine if Bacteroides gingivalis fibrinogen binding protein is an in vivo virulence factor in the cynomolgus monkey model of ligature-induced periodontitis by: (1) determining if active immunity to B. gingivalis FBP (a) prevents emergence of B. gingivalis or other Bacteroides species in the microbiota of developing periodontitis lesions, or (b) prevents development of periodontitis and (2) determining if B. gingivalis mutants lacking fibrinogen binding protein (FBP-) can implant and emerge in the microbiota of developing periodontitis lesions.

某些形式的牙龈炎和牙周炎与 土著黑色素 龈下微生物群中的拟杆菌属，特别是 牙龈拟杆菌和中间拟杆菌。 不 知道哪些毒力因子或因子允许出现 这些有机体，或者这些有机体的出现 有助于牙龈炎和牙周炎的发病机制。 数个B。牙龈炎和B.中间体菌株结合， 体外粘附纤维蛋白原和纤维蛋白。 粘合和粘附 这些菌株对纤维蛋白原的作用是由细菌蛋白（Mr 200 k）。 建议研究纤维蛋白原是否 结合蛋白（FBP）是一种体内毒力因子， B的出现。牙龈炎在发展中的牙周炎病变， 食蟹猴 阐明 拟杆菌的出现对于理解 牙周病的病因作用。 了解 这些机制对于实现长期目标也是必要的 这项研究的目的是制定策略来阻止 这些生物体，从而阻止发展， 牙周炎 建议的具体目标如下： 1. 目的从人血浆中分离纯化纤维蛋白原结合蛋白， 牙龈拟杆菌和拟杆菌的食蟹猴菌株 中间体 2. 为了表征和比较纤维蛋白原结合蛋白， 牙龈拟杆菌的猴和人菌株， 中间拟杆菌 3. 确定牙龈拟杆菌是否与纤维蛋白原结合 蛋白是食蟹猴体内的毒力因子 结扎诱导的牙周炎模型： (1)确定是否对B有主动免疫。牙龈FBP (a)防止B的出现。牙龈菌或其他拟杆菌 在牙周炎病变的微生物群中的物种， 或 (b)预防牙周炎的发展， (2)确定是否B.缺乏纤维蛋白原结合的牙龈炎突变体 蛋白质（FBP-）可以植入并出现在微生物群中， 发展成牙周炎损伤。