ATTACHMENT PROTEIN INTERACTION WITH PERIODONTAL BACTERIA

附着蛋白与牙周细菌的相互作用

• 批准号: 3447141
• 负责人: MARILYN S LANTZ
• 金额: $ 4.9万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-09-01 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3447141
• 关键词: Actinomyces; Bacteroides; Fusobacterium; fibrinogen; gene expression; ligands; oral bacteria; periodontitis; periodontium; physical chemical interaction

Considerable evidence suggests that periodontitis, the major cause of tooth loss in the world, is initiated and perpetuated by members of the oral microflora. The long-term objective of this proposal is to assess the role of host attachment proteins, including fibronectin, fibrinogen and laminin as modulators of host colonization by suspected periodontal pathogens. These attachment proteins bind bacteria, host cells and a variety of ligands and may serves as "linker" molecules between bacteria and host tissue cells. Studies described are directed toward eludicating the nature of attachment protein-bacterial interactions. The kinetics and specificity of laminin binding to Bacteroides gingivalis (W) will be determined, including purification of the Bacteroides gingivalis (W) laminin receptor. Bacteroides gingivalis strains, other oral Bacteroides species and a variety of suspected periodontopathic bacteria will be assessed for the ability to bind fibronectin, fibrinogen and laminin. These survey studies will lead to purification and characterization of selected attachment protein receptors. Information relative to the nature of attachment protein-bacterial interaction may be critical to understanding colonization of the periodontal pocket by specific members of the indigenous oral microflora and may provide mechanisms for selectively interfering with such host colonization by pathogens.

大量证据表明，牙周炎，牙齿的主要原因， 世界上的损失，是由口头成员发起和延续的。 微生物群落 本建议的长期目标是评估 包括纤维连接蛋白、纤维蛋白原和层粘连蛋白 作为疑似牙周病原体的宿主定殖的调节剂。 这些附着蛋白结合细菌、宿主细胞和各种 配体，并可作为细菌和宿主之间的“接头”分子 组织细胞 所描述的研究是针对逃避依恋的性质 蛋白质-细菌相互作用 层粘连蛋白的动力学和特异性 将测定与牙龈拟杆菌（W）的结合，包括 牙龈拟杆菌（W）层粘连蛋白受体的纯化。 牙龈拟杆菌菌株、其他口腔拟杆菌种和 各种可疑的牙周病细菌将进行评估， 结合纤连蛋白、纤维蛋白原和层粘连蛋白的能力。 这些调查研究 将导致所选附着物的纯化和表征 蛋白质受体 与附着蛋白质性质相关的信息-细菌 相互作用可能是理解殖民的关键 牙周袋由特定成员的土著口腔微生物 并且可以提供用于选择性地干扰这种宿主的机制 病原体的定植。