DISTRIBUTION AND ULTRASTRUCTURE OF DENTAL INNERVATION

牙齿神经分布和超微结构

基本信息

批准号：
3219260
负责人：
MARGARET READ BYERS
金额：
$ 16.64万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
起止时间：
1979-05-01 至 1994-04-30
项目状态：
已结题

项目摘要

The overall objective is to improve our understanding of the sensory neurobiology of normal and pathologic dental tissue as a basis for improved dental diagnosis and treatment. Immunocytochemical studies in rats are designed to examine the structure and cytochemistry of normal dental innervation; to analyze neural reactions to dental injury and inflammation, including interactions between nerve fibers, immune cells, and pulpal healing rates; to study the mechanisms of peripheral nerve sprouting in relation to growth factors, nerve impulse inhibitors, or extracellular matrix molecules; and to correlate peripheral sprouting with possible alterations of the affected trigeminal axons and nerve cell bodies. In addition, physiological studies of axons in trigeminal nerves of ferrets and cats will examine possible changes in sensory and anesthetic properties of inflamed teeth. Finally, immunocytochemistry of normal extracted human teeth will be compared with the animal studies. For normal rat dental innervation we will continue immunocytochemical LM and EM analyses of various neuropeptides, transmitters and enzymes to gain better information on the types of nerve fibers innervating teeth and periodontium, their receptor distribution, their support cell associations, and whether or not they are immunoreactive for nerve growth factor receptor. For studies of nerve sprouting, three types of injury to rat molars will be done: shallow cavities; small pulp exposures causing limited pulpitis; and large occlusal exposures causing irreversible pulpitis. The effects of sensory or sympathetic denervation on surviving nerve fibers, on immune cell invasion and on pulpal healing rates will be analyzed for the three injuries, as will the effects of anti-inflammatory drugs. To study mechanisms of dental nerve sprouting, slow release carriers containing growth factors, tetrodotoxin, or laminin will be implanted into rat molars, and the degree of sprouting measured in relation to pulpal and immune cell responses. In some cases retrograde transport of WGA-HRP or I 125- NGF will be done to allow specific immunocytochemical studies of the cell bodies of the affected nerve fibers. In ferrets and cats we will induce inflammation in one canine tooth and then record from single fibers in alveolar nerve to analyze sensory properties of A and C fibers and their response to local anesthesia compared with contralateral control teeth. These experimental studies of normal and pathologic dental innervation will have significance for somatosensory neurobiology, neuro-immune interaction, neural plasticity, basic mechanisms of inflammation and healing, and diagnosis and treatment of dental pain.

总体目标是提高我们对正常和病理牙科组织的感觉神经生物学作为一种改善牙齿诊断和治疗的基础。大鼠的免疫细胞化学研究旨在检查正常牙齿神经的结构和细胞化学；到分析对牙齿损伤和炎症的神经反应，包括神经纤维，免疫细胞和牙髓愈合率；研究周围神经的机制与生长因子，神经冲动抑制剂有关的发芽，或细胞外基质分子；并关联外围发芽，可能会改变受影响的三叉状轴突和神经细胞体。另外，生理研究雪貂和猫的三叉神经中的轴突将检查发炎的感觉和麻醉特性的可能变化牙齿。最后，正常提取人的免疫细胞化学牙齿将与动物研究进行比较。对于正常的大鼠牙科支配，我们将继续免疫细胞化学LM和EM各种神经肽的分析，发射机和酶以获取有关类型的更好信息神经支配牙齿和牙周的神经纤维，其受体分发，其支持单元关联以及是否它们对神经生长因子受体具有免疫反应性。为了神经发芽的研究，大鼠磨牙的三种损伤将完成：浅腔；小纸浆暴露引起有限的牙髓炎；和大型咬合暴露导致不可逆的牙髓炎。感觉或交感神经对在免疫细胞入侵和牙髓上存活的神经纤维，这三个受伤将分析治疗率，抗炎药的作用。研究牙齿的机制神经发芽，含有生长因子的缓慢释放载体，四毒素或层粘连蛋白将植入大鼠磨牙，然后将其植入与牙髓和免疫细胞相关的发芽程度回答。在某些情况下，WGA-HRP或I 125-的逆行运输 NGF将进行以允许特定的免疫细胞化学研究受影响的神经纤维的细胞体。在雪貂和猫中我们将在一个犬牙中诱发炎症，然后记录从肺泡神经的单纤维到分析感觉特性 A和C纤维及其对局部麻醉的反应与对侧对照牙齿。这些实验研究正常和病理牙齿神经神经对体感神经生物学，神经免疫相互作用，神经可塑性，炎症和愈合的基本机制，以及诊断和治疗牙齿疼痛。