权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Novel enzyme diversity for improving cleaning and hygiene

用于改善清洁和卫生的新型酶多样性

基本信息

批准号：
BB/N023838/1
负责人：
Jennifer Littlechild
金额：
$ 12.72万
依托单位：
UNIVERSITY OF EXETER
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2016
资助国家：
英国
起止时间：
2016 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FN023838%2F1
关键词：
Novel enzyme diversity improving cleaning

项目摘要

The human body produces up to gram quantities each day of sebum that is deposited on our clothes. The commonenzymes added to many washing powders are protease and lipase enzymes to digest the proteins and lipids on the washfabric. The sebum contains a complex mixture of free fatty acids, triglycerides and cholesterol esters, wax esters, andsqualene. much of which remains on the clothes after the wash. In addition there is a need to remove resistant stains suchas tea stains which are difficult to remove without some bleaching of the fabric.The are other enzyme activities which exist in nature that can breakdown these other products and also enzymes whichhave a bleaching effect.The project will aim to identify and characterise these enzymes from a large DNA resource that has been isolated from hotenvironments around the globe. This resource can be mined using bioinformatic techniques to identify enzyme classes thatwill tackle these cleaning problems. This will allow the production of small amounts of the new robust enzymes to beproduced for testing at the partner company, Unilever. In addition libraries of small pieces of DNA from differentthermophilic genomes have be made as libraries that can be screening for activity of the enzymes again specific fatty acidsetc. The overall project will allow access to novel stable new enzymes that could be used to improve the overall cleaningprocess.The use of enzymes in these processes is environmentally friendly and avoids the use of harsh bleaching agents.

人体每天产生多达克的皮脂沉积在我们的衣服上。添加到许多洗衣粉中的常见酶是蛋白酶和脂肪酶，以消化洗涤织物上的蛋白质和脂质。皮脂含有游离脂肪酸、甘油三酯和胆固醇酯、蜡酯和角鲨烯的复杂混合物。洗涤后大部分残留在衣服上。此外，还需要去除顽固的污渍，如茶渍，这些污渍在不漂白织物的情况下很难去除。这些是自然界中存在的其他酶活性，可以分解这些其他产物，也可以是具有漂白作用的酶。该项目的目标是从地球仪中分离出的大量DNA资源中鉴定和纯化这些酶。这种资源可以利用生物信息学技术来挖掘，以确定将解决这些清洁问题的酶类。这将允许生产少量的新的强大的酶被生产用于在合作伙伴公司联合利华进行测试。此外，来自不同嗜热基因组的小片段DNA文库已经被制成文库，可以筛选酶的活性和特定的脂肪酸等。整个项目将允许获得新的稳定的新酶，可用于改善整个清洁过程。在这些过程中使用酶是环境友好的，避免使用苛刻的漂白剂。