Novel enzyme diversity for improving cleaning and hygiene

人体每天产生多达克的皮脂，沉积在我们的衣服上。许多洗衣粉中添加的常见酶是蛋白酶和脂肪酶，用于消化织物上的蛋白质和脂质。皮脂含有游离脂肪酸、甘油三酯和胆固醇酯、蜡酯和角鲨烯的复杂混合物。洗后衣服上还残留着很多。此外，还需要去除顽固污渍，如茶渍，如果不对织物进行一些漂白，这些污渍很难去除。自然界中存在的其他酶活性可以分解这些其他产物，还有具有漂白作用的酶。该项目旨在从从全球高温环境中分离出来的大量DNA资源中识别和表征这些酶。这种资源可以使用生物信息学技术来识别酶类，以解决这些清洁问题。这将允许生产少量新的强效酶，以供合作伙伴联合利华公司进行测试。此外，来自不同嗜热基因组的小片段DNA文库已被制成文库，可用于筛选酶的活性，再次是特定脂肪酸。整个项目将允许获得新的稳定的新酶，可用于改善整个清洁过程。在这些过程中使用酶是环保的，避免了使用苛刻的漂白剂。

The human body produces up to gram quantities each day of sebum that is deposited on our clothes. The commonenzymes added to many washing powders are protease and lipase enzymes to digest the proteins and lipids on the washfabric. The sebum contains a complex mixture of free fatty acids, triglycerides and cholesterol esters, wax esters, andsqualene. much of which remains on the clothes after the wash. In addition there is a need to remove resistant stains suchas tea stains which are difficult to remove without some bleaching of the fabric.The are other enzyme activities which exist in nature that can breakdown these other products and also enzymes whichhave a bleaching effect.The project will aim to identify and characterise these enzymes from a large DNA resource that has been isolated from hotenvironments around the globe. This resource can be mined using bioinformatic techniques to identify enzyme classes thatwill tackle these cleaning problems. This will allow the production of small amounts of the new robust enzymes to beproduced for testing at the partner company, Unilever. In addition libraries of small pieces of DNA from differentthermophilic genomes have be made as libraries that can be screening for activity of the enzymes again specific fatty acidsetc. The overall project will allow access to novel stable new enzymes that could be used to improve the overall cleaningprocess.The use of enzymes in these processes is environmentally friendly and avoids the use of harsh bleaching agents.