Advanced cryobanking for recalcitrant-seeded Australian rainforest plants

该项目旨在发展一个跨学科的研究联盟，以提高顽固的澳大利亚热带雨林植物物种的生态恢复效率。该项目希望提供基础知识，以针对顽固种子本地物种超低温保存的两个主要障碍：相对较大的干燥敏感性，以及活跃的新陈代谢和早熟萌发。这些属性严重限制了有效利用收集的种质进行长期储存的时间。生物化学、生物物理和分子模拟方法的组合将用于优化各种濒危澳大利亚热带雨林物种的超低温保存。这应该会带来显著的好处，例如使保护机构能够极大地增强其保护独特的澳大利亚雨林植物的能力，特别是考虑到目前由于栖息地丧失和全球变暖造成的威胁。

This project aims to develop an interdisciplinary research alliance to enhance the efficiency of ecological restoration for recalcitrant Australian rainforest plant species. The project expects to provide fundamental knowledge to target two major impediments to cryostorage of recalcitrant-seeded native species: desiccation sensitivity with relatively large size, and active metabolism and precocious germination. These attributes severely limit the time available for effective use of collected germplasm for long-term storage. A combination of biochemical, biophysical and molecular simulation approaches will be used to optimise cryopreservation of a wide range of endangered Australian rainforest species. This should provide significant benefits such as enabling conservation agencies to greatly enhance their ability to preserve the unique Australian rainforest flora, particularly given current threats due to habitat loss and global warming.