Feasibility of large-scale oocyte cryobanking of C57BL/6N mice and the influence of donor microbiome on recovery and genomic stability

C57BL/6N 小鼠大规模卵母细胞冷冻银行的可行性以及供体微生物组对恢复和基因组稳定性的影响

• 批准号: 10610678
• 负责人: CRAIG L FRANKLIN
• 金额: $ 45.33万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10610678
• 关键词: Anaphase; Aneuploidy; Animals; Archives; Breeding; C57BL/6 Mouse; C57BL/6N Mouse; Charge; Chromosome abnormality; Chromosomes; Communities; Competence; Cryopreservation; Data; Derivation procedure; Development; Disease model; Embryo; Embryo Transfer; Embryonic Development; Equus caballus; Estrus; Evaluation; Event; Female; Fertilization in Vitro; Follicle Stimulating Hormone; Generations; Genetic; Genetic Drift; Genetically Engineered Mouse; Genome; Genome Stability; Genomics; Germ Cells; Goals; Gonadotropins; Haploidy; In Vitro; Live Birth; Logistics; Metabolic; Metagenomics; Metaphase; Methods; Microtubules; Missouri; Mitochondrial DNA; Modeling; Mouse Strains; Mus; Mutant Strains Mice; Oocytes; Outcome; Phenotype; Physiological; Play; Population; Pregnancy loss; Procedures; Production; Publications; Recovery; Research; Research Personnel; Resources; Resuscitation; Rivers; Role; Savings; Serum; Shipping; Ships; Sperm Banks; Superovulation; Surrogate Mothers; Testing; Time; Transgenic Mice; Universities; Variant; Vendor; Weather; blastocyst; cost; cryobiology; embryo cryopreservation; embryo quality; experience; experimental study; genetic strain; genome sequencing; gut microbiota; human error; improved; in vivo; inhibin; meetings; microbial; microbiome; mouse model; oocyte cryopreservation; oocyte quality; operation; preservation; pup; response; segregation; sperm cell; sperm cryopreservation; success; tool; transcriptomics; whole genome

Project Summary The goal of this project is to establish large-scale oocyte cryopreserve and assess the viability, cost and animal savings, as well as determine what factors may influence the recovery of viable rederived mice. Our experience in cryopreservation has shown varying quality of recovered oocytes among different vendors, even within a specific mouse strain. We will cryopreserve oocytes from C57BL/6N mice from the four most commonly used vendors (Jackson, Charles River, Taconic and Envigo). We compare the quality of oocytes through IVF to the blastocyst stage as well as to live birth. We will also examine possible corollaries to embryo quality including genome aneuploidy and the microbiome of the donor dams. This data will inform our implementation of large-scale preservation and rederivation and identify factors that influence successful outcomes. This proposal will lead to efficiencies in cryopreservation of haploid gametes (oocytes) and success would; i) reduce costs related animal shipments required on demand of a project ii) would eliminate the requirement to synchronize oocyte production and ET recipient mice’s estrus cycle, iii) enable testing of each batch of oocytes for developmental competency, iv) improve successful live pup production rates and reduce the time between when a researcher orders an IVF recovery and we can provide animals, v) and collectively, the use of cryopreserved oocytes will reduce the operating expenses of MMRRC. This information will be shared with the other three MMRRCs either during our monthly consortium meetings or during our annual consortium meeting. Additionally, this information will be shared widely through publications and presentations.

项目总结