Advanced cryobanking for propagation-recalcitrant and critically endangered plant species

针对难繁殖和极度濒危植物物种的先进冷冻库

• 批准号: LP140100993
• 负责人: Prof Ricardo Mancera
• 金额: $ 31.75万
• 依托单位: Curtin University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-02-26 至 2018-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP140100993
• 关键词: Advanced; cryobanking; propagation; recalcitrant; critically

This project aims to advance methods for the conservation of recalcitrant/threatened plant species to achieve best practice ecological restoration in areas of high biodiversity. In particular, the nature of freezing and dehydration damage to cell membranes at various stages of tissue culture and cryopreservation will be investigated to try to minimise deleterious effects. In addition, metabolic changes that affect cryogenic survival will be assessed to provide further insight into the role of oxidative stress and the toxicity of cryopreservation processes. Ultimately, improved cryogenic protocols will be developed to maintain the integrity of long-term cryobanks of key species for ecological restoration, such as in post-mined areas.

该项目旨在推进顽固/受威胁植物物种的保护方法，以在生物多样性丰富的地区实现生态恢复的最佳实践。特别是，将研究组织培养和冷冻保存各个阶段冷冻和脱水对细胞膜造成的损害，以尽量减少有害影响。此外，还将评估影响低温存活的代谢变化，以进一步了解氧化应激的作用和低温保存过程的毒性。最终，将开发改进的低温协议，以保持关键物种长期低温库的完整性，以进行生态恢复，例如在采矿后地区。