REGULATION OF INDIVIDUAL NEPHRON FILTRATION RATES

单个肾单位滤过率的调节

• 批准号: 3225574
• 负责人: WILLIAM H DANTZLER
• 金额: $ 11.34万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3225574
• 关键词: glomerular filtration; hormone regulation /control mechanism; kidney circulation; kidney disorder diagnosis; kidney metabolism; prolactin; radiotracer; renal tubular transport; saluresis; vasopressins

Our long-term objective is to increase our understanding of the regulation of nephron function by exploiting the unique renal structure of birds, the highly specialized renal structure of certain desert rodents, and the specialized transport properties of the nephrons of reptiles. The avian kidney has a population of nephrons resembling reptilian nephrons that function independently of each other, do not contribute directly to the concentrating mechanism, and can cease filtering altogether under some circumstances and a population of nephrons resembling mammalian nephrons that function together in the concentrating mechanism, do not normally cease filtering, but may alter their filtration rates. The kidneys of certain tiny desert rodents have juxtamedullary nephrons that are several times larger than the superficial cortical nephrons and a very long papilla and represent an intermediate state between the avian kidney and the more commonly studied mammalian kidneys. In contrast to mammalian nephrons, reptilian and avian nephrons secrete phosphate under the control of parathyroid hormone. Our primary goals under this broad objective involve studies of: 1) the regulation of nephron function, including the regulation of single nephron filtration rates (SNGFR) and tubular transport, in avian, desert rodent, and reptilian kidneys; 2) the relationship of single nephron function to the concentrating and dilut ng mechanism; and 3) the patterns and regulation of renal portal flow within the avian kidney. We are performing and plan to continue micropuncture and microperfusion (both in vivo and in vitro) studies of specific factors regulating SNGRFs (e.g., sodium, chloride, antidiuretic hormone, and distal tubule-glomerular feedback) and of factors influencing tubular transport, including phosphate transport (e.g., parathyroid hormone). We also plan micropuncture studies of loop of Henle function and the effects of alterations in SNGFR on this function. Electron microprobe analysis is being used to permit measurement of multiple inorganic ions in the tiny samples obtained. We also plan in vivo studies of avian renal portal blood flow patterns by silicone injection techniques and micropressure measurements and in vitro studies of specific factors (e.g., antidiuretic hormone) regulating the renal portal valve.

我们的长远目标是加深我们对这项规定的了解。 通过利用鸟类独特的肾脏结构， 某些沙漠啮齿动物高度专门化的肾脏结构，以及 爬行动物肾脏的特殊运输特性。那只鸟 肾脏有一群类似爬行动物肾脏的 功能相互独立，不会直接对 浓缩机制，并可以在某些情况下完全停止过滤 环境和类似哺乳动物肾单位的肾单位数量 在浓缩机制中共同发挥作用，通常不会 停止过滤，但可能改变它们的过滤速度。中国人的肾脏 某些微小的沙漠啮齿动物有几个相邻的延髓肾单位 比皮质浅层肾单位和一个很长的乳突大 代表了禽类肾脏和更多 普遍研究的哺乳动物肾脏。与哺乳动物的肾单位不同， 爬行动物和鸟类肾脏在控制下分泌磷酸盐 甲状旁腺激素。我们在这个大目标下的主要目标包括 研究：1)肾单位功能的调节，包括 单肾单位滤过率(SNGFR)和肾小管的调节 在鸟类、沙漠啮齿动物和爬行动物肾脏中的转运；2) 单肾单位功能与浓缩稀释的关系 3)肾内门脉血流的模式和调节。 鸟肾。我们正在进行并计划继续进行显微穿刺术和 特定因子的微灌注(体内和体外)研究 调节SNGRF(例如，钠、氯化物、抗利尿激素和远端 肾小管-肾小球反馈)和影响肾小管转运的因素， 包括磷酸盐运输(例如甲状旁腺激素)。我们还计划 汉勒功能环的显微穿刺术及针刺对其影响的研究 SNGFR中对此函数的更改。电子探针分析是 用来测量微米中的多种无机离子 所获得的样本。我们还计划对禽类肾脏门静脉血进行体内研究。 有机硅注射技术的流型与微压 特定因子(例如，抗利尿剂)的测量和体外研究 激素)调节肾门静脉瓣膜。