BIOCHEMICAL ASPECTS OF RENAL ELECTROLYTE TRANSPORT

肾脏电解质转运的生化方面

• 批准号: 3226772
• 负责人: IRWIN M WEINER
• 金额: $ 15.91万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-09-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3226772
• 关键词: 2,4 dinitrophenol; active transport; adenosinetriphosphatase; calcium; chlorothiazide; cyclic AMP; diuretics; drug metabolism; electrolytes; gout drug; heart; ion transport; kidney metabolism; kidney pharmacology; organic acid; organic base; p aminohippurate; phenytoin; potassium; probenecid; pyrazinamide; renal tubular transport; stop flow technique; urate; urinary tract pharmacology; vasodilators; vitamin D

This proposal is for the study of the renal disposition of drugs. The actions of drugs on the kidney, and drug interactions. The subprojects are: 1) A study of the active reabsorptive mechanism(s) for drugs. The questions addressed are: Can mutual inhibition of transport be demonstrated among the various drugs which are actively reabsorbed? Is this reabsorptive mechanism the same as the mechanism for uric acid? Can active reabsorptive fluxes be demonstrated for uricosuric drugs which do not seem to be reabsorbed in clearance experiments? 2) The uricosuric action of zoxazolamine. The question is: Can an organic base per se be uricosuric or must it be converted in vivo to an anionic metabolite. 3) The hypocalicuric action of chlorothiazide. The question: Is the hypocalcuric action a result of inhibition of the enzyme(s) phosphodiesterase? 4) A macromolecular inhibitor of carbonic anhydrase. The question: What is the quantitative role of lumenal carbonic anhydrase in the reabsorption of bicarbonate? The experiments will be conducted in dogs, chimpanzees, Cebus monkeys and rats. The major techniques are renal clearance, stop-flow, micropuncture, microperfusion.

该提案旨在研究药物的肾脏处置。 行动 药物对肾脏的影响以及药物相互作用。 子项目是：1）研究 药物的主动重吸收机制。 解决的问题是： 不同药物之间是否可以证明转运的相互抑制作用 哪些是主动重吸收的？这种重吸收机制与 尿酸的作用机制？可以证明活性重吸收通量 在清除实验中似乎不会被重吸收的促尿酸排泄药物？ 2）佐恶唑明的促尿酸排泄作用。 问题是：有机物可以吗？ 碱本身具有促尿酸排泄作用，或者必须在体内转化为阴离子 代谢物。 3）氯噻嗪的低钙尿作用。 问题： 是 酶抑制导致的低尿钙作用 磷酸二酯酶？ 4) 碳酸酐酶大分子抑制剂。 这 问题： 腔内碳酸酐酶在体内的定量作用是什么？ 碳酸氢盐的重吸收？ 实验将在狗、黑猩猩、宿雾猴和老鼠身上进行。 主要技术有肾清除、停流、微穿刺、 微灌注。