BIOCHEMICAL ASPECTS OF RENAL ELECTROLYTE TRANSPORT

肾脏电解质转运的生化方面

• 批准号: 3151314
• 负责人: IRWIN M WEINER
• 金额: $ 16.06万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-09-01 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151314
• 关键词: 2,4 dinitrophenol; active transport; adenosinetriphosphatase; calcium; chlorothiazide; cyclic AMP; diuretics; drug metabolism; electrolytes; gout drug; heart; ion transport; kidney metabolism; kidney pharmacology; organic acid; organic base; p aminohippurate; phenytoin; potassium; probenecid; pyrazinamide; renal tubular transport; stop flow technique; urate; urinary tract pharmacology; vasodilators; vitamin D

This proposal is for the study of the renal disposition of drugs. The actions of drugs on the kidney, and drug interactions. The subprojects are: 1) A study of the active reabsorptive mechanism(s) for drugs. The questions addressed are: Can mutual inhibition of transport be demonstrated among the various drugs which are actively reabsorbed? Is this reabsorptive mechanism the same as the mechanism for uric acid? Can active reabsorptive fluxes be demonstrated for uricosuric drugs which do not seem to be reabsorbed in clearance experiments? 2) The uricosuric action of zoxazolamine. The question is: Can an organic base per se be uricosuric or must it be converted in vivo to an anionic metabolite. 3) The hypocalicuric action of chlorothiazide. The question: Is the hypocalcuric action a result of inhibition of the enzyme(s) phosphodiesterase? 4) A macromolecular inhibitor of carbonic anhydrase. The question: What is the quantitative role of lumenal carbonic anhydrase in the reabsorption of bicarbonate? The experiments will be conducted in dogs, chimpanzees, Cebus monkeys and rats. The major techniques are renal clearance, stop-flow, micropuncture, microperfusion.

这项建议是为了研究药物的肾脏处置。行动 肾脏上的药物，以及药物的相互作用。这些子项目包括：1)研究 对药物的主动重吸收机制的研究(S)。所提出的问题如下： 不同药物之间是否存在相互抑制转运的现象？ 哪些是积极重新吸收的？这种再吸收机制是否与 尿酸的作用机制？活性重吸收通量能被证明为 尿酸类药物在清除实验中似乎不会被重新吸收？ 2)唑唑胺的尿酸作用。问题是：有机食品能否 碱本身是尿酸的，还是必须在体内转化为阴离子 代谢物。3)氯噻嗪的降钙作用。问题是： 由于酶的抑制而产生的低热量作用(S) 磷酸二酯酶？4)碳酸酐酶的大分子抑制剂。这个 问：腔内碳酸酐酶在体内的定量作用是什么？ 重吸收重碳酸盐？ 这些实验将在狗、黑猩猩、Cebus猴子和大鼠身上进行。 主要技术有肾清除、阻断血流、显微穿刺术、 微灌注术。

项目成果

Renal excretion of urate: factors determining the actions of drugs.

尿酸盐的肾脏排泄：决定药物作用的因素。

• DOI: 10.1038/ki.1980.184
• 发表时间: 1980
• 期刊: Kidney international
• 影响因子: 19.6
• 作者: Roch-Ramel,F;Weiner,IM
• 通讯作者: Weiner,IM

Simple, rapid high-performance liquid chromatographic determination of bupivacaine in human plasma.

简单、快速的高效液相色谱法测定人血浆中的布比卡因。

• DOI: 10.1016/0378-4347(87)80115-9
• 发表时间: 1987
• 期刊: Journal of chromatography
• 作者: Sullivan,NW;Weiner,L
• 通讯作者: Weiner,L

Effects of p-aminohippurate and pyrazinoate on urate excretion in Cebus monkeys.

对氨基马尿酸盐和吡嗪酸盐对宿雾猴尿酸盐排泄的影响。

• 发表时间: 1984
• 期刊: The Journal of pharmacology and experimental therapeutics
• 作者: Perez-Gonzalez,M;Weiner,IM
• 通讯作者: Weiner,IM

Medullary cystic disease: an inherited form of autoimmune interstitial nephritis?

髓样囊性病：自身免疫性间质性肾炎的遗传形式？

• DOI: 10.1016/s0272-6386(87)80108-7
• 发表时间: 1987
• 期刊: American journal of kidney diseases : the official journal of the National Kidney Foundation
• 作者: Kelly,CJ;Neilson,EG
• 通讯作者: Neilson,EG

Effects of pyrazinoate and p-aminohippurate on renal urate excretion by the dog and guinea pig.

吡嗪酸盐和对氨基马尿酸盐对狗和豚鼠肾脏尿酸盐排泄的影响。

• 发表时间: 1983
• 期刊: The Journal of pharmacology and experimental therapeutics
• 作者: Perez-Gonzalez,M;Weiner,IM
• 通讯作者: Weiner,IM