CELL RESPONSE TO MODIFIED TI SURFACES

细胞对改性 TI 表面的反应

• 批准号: 3222304
• 负责人: JOHN C. KELLER
• 金额: $ 15.17万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3222304
• 关键词: acidity /alkalinity; alkaline phosphatase; alloys; biomaterial development /preparation; biomaterial evaluation; cell adhesion; collagen; computer simulation; connective tissue cells; electron probe spectrometry; endosseous dental implant; extracellular matrix; fibroblasts; gene expression; laboratory rat; mathematical model; metal dental material; monoclonal antibody; osteoblasts; osteocalcin; osteonectin; oxides; radiotracer; scanning electron microscopy; surface property; titanium; wound healing

Dental implants fabricated form Ti and, T--6A1-4V alloy are widely used in clinical practice, yet there is no consensus or established criterion regarding the design or fabrication of implant surfaces. As a result there is little information currently available concerning specific biological responses, such as deposition of extracellular matrix molecules and attachment of cells, which occur during the initial stages of wound healing at the intimate implant and hard and soft tissue interfaces. The overall objective of this research proposal is to investigate some of the cell responses to both standard, commercially available implant surfaces as well as modified Ti based implant surfaces. Preliminary data from our lab suggests that available implant systems vary widely in at least their surfaces topography and that molecular interactions and attachment of cells at these surfaces are affected by the nature of the substrate. The experiments proposed in this application are specifically designed to study a number of variables by surface characterization techniques including scanning electron microscopy (SEM/EDAX), electron spectroscopy for chemical analysis (ESCA), auger electron spectroscopy (AES) and surface energy (contact angle) measurements. The effects of variables such a type of metal, surface topography, oxide structure and composition and surface charge and energy on fundamental biological events such as matrix adhesion, and cellular attachment, spreading and proliferation on these surfaces will be ascertained. We hypothesize that chemical and biochemical modifications of the implant surface will result in enhanced biological acceptance and long term tissue integration. This type of research has far reaching clinical implications in that it will define a model implant surface which can foster improved tissue reactions, thereby potentially decreasing the long healing periods now necessary with most commercial implant systems.

由Ti和T-6Al-4V合金制成的牙科植入物被广泛使用 在临床实践中，尚未达成共识或建立标准 关于植入物表面的设计或制造。 因此 目前关于具体的信息很少。 生物反应，如细胞外基质的沉积 分子和细胞的附着，这发生在初始阶段 在亲密植入物和硬软组织的伤口愈合 接口。 这项研究的总体目标是调查一些 的细胞反应， 表面以及改性钛基植入物表面。 初步 我们实验室的数据表明，可用的植入系统差异很大， 至少它们的表面形貌和分子间的相互作用 细胞在这些表面上的附着受到细胞的性质的影响。 衬底 本申请中提出的实验是专门设计的 通过表面表征技术研究一些变量 包括扫描电子显微镜（SEM/EDAX）、电子光谱 用于化学分析（ESCA）、俄歇电子能谱（AES）和 表面能（接触角）测量。 变量的影响 这种类型的金属、表面形貌、氧化物结构 基本生物化学的组成、表面电荷和能量 例如基质粘附和细胞附着、扩散和 将确定这些表面上的增殖。 我们假设 植入物表面的化学和生物化学修饰将 导致增强的生物接受性和长期组织 一体化 这种类型的研究具有深远的临床意义， 将定义可以促进改善组织的模型植入物表面 反应，从而可能减少现在的长愈合期 这是大多数商业植入系统所必需的。