INTRACELLULAR REACTIONS IN GASTRIC MUCOSA

胃粘膜的细胞内反应

• 批准号: 3225293
• 负责人: STEPHEN J HERSEY
• 金额: $ 15.63万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-12-01 至 1989-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3225293
• 关键词: acetylcholine; acidity /alkalinity; adenosine diphosphate; cell membrane; cellular pathology; cellular respiration; cyclic AMP; cytochromes; fluorimetry; gastric mucosa; gastrins; histamine; hydrochloric acid; ion transport; membrane permeability; mitochondria; mitochondrial membrane; nicotinamide adenine dinucleotide; oxidation reduction reaction; oxidative phosphorylation; secretion; spectrometry; stomach

The longterm goal of this program is to describe the sequence of cellular reactions which lead to the secretion of hydrochloric acid by the gastric mucosa with emphasis on those reactions which serve as the major controlling steps. Recent interest in this area derives from the success of antisecretory agents in the clinical management of ulcer disease and the appreciation of the important role of proton transport in various biological processes. The current proposal seeks to extend previous observations concerning the cellular control of acid secretion so as to provide a more detailed understanding of the control mechanisms. Projects will utilize the recently developed preparations of isolated gastric glands and digitonin-permeabilized cells as well as the more traditional amphibian gastric mucosa as in vitro models. A combination of modern biophysical, biochemical, and morphological techniques will be used to investigate selected aspects of cellular control mechanisms. The major areas to be investigated include: 1) regulation of histamine H2-receptor; 2) the role of protein kinases, specifically the cAMP-dependent and calcium/phospholipid-dependent enzymes; 3) the basic mechanisms involved in the morphological transition of the parietal cell which occurs with stimulation; 4) the relationship between mitochondrial metabolism and proton transport; and 5) the effects of omeprazole, an H-KATPase inhibitor, on ion transport mechanisms. These areas are identified as important regulatory sites, the investigation of which are timely and promises a high rate of progress towards the longterm goal.

这个程序的长期目标是描述细胞的序列 导致胃分泌盐酸的反应 粘膜，强调作为主要反应的那些反应 控制步数。最近对这一领域的兴趣来自于 抗分泌剂在溃疡病临床治疗中的应用 认识到质子输运在各种环境中的重要作用 生物过程。 目前的建议旨在扩大先前关于 从而为细胞对酸分泌的控制提供更详细的 对控制机制的理解。项目将利用 新近开发的离体胃腺体制剂和 洋地黄素渗透性细胞以及更传统的两栖动物 胃粘膜作为体外模型。现代生物物理学、 生化和形态技术将被用来研究 细胞控制机制的精选方面。主要领域包括 研究包括：1)组胺H2受体的调节；2)组胺H2受体的作用 蛋白激酶，特别是cAMP依赖和 钙/磷脂依赖的酶；3)涉及的基本机制 顶叶细胞的形态转变 4)线粒体代谢与刺激的关系。 质子转运；以及5)H-KATPase抑制剂奥美拉唑的作用。 关于离子传输机制。这些领域被认为是重要的 监管网站，调查及时，承诺高 实现长期目标的进度。