INTRACELLULAR REACTIONS IN GASTRIC MUCOSA
胃粘膜的细胞内反应
基本信息
- 批准号:3225293
- 负责人:
- 金额:$ 15.63万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1976
- 资助国家:美国
- 起止时间:1976-12-01 至 1989-11-30
- 项目状态:已结题
- 来源:
- 关键词:acetylcholine acidity /alkalinity adenosine diphosphate cell membrane cellular pathology cellular respiration cyclic AMP cytochromes fluorimetry gastric mucosa gastrins histamine hydrochloric acid ion transport membrane permeability mitochondria mitochondrial membrane nicotinamide adenine dinucleotide oxidation reduction reaction oxidative phosphorylation secretion spectrometry stomach
项目摘要
The longterm goal of this program is to describe the sequence of cellular
reactions which lead to the secretion of hydrochloric acid by the gastric
mucosa with emphasis on those reactions which serve as the major
controlling steps. Recent interest in this area derives from the success
of antisecretory agents in the clinical management of ulcer disease and the
appreciation of the important role of proton transport in various
biological processes.
The current proposal seeks to extend previous observations concerning the
cellular control of acid secretion so as to provide a more detailed
understanding of the control mechanisms. Projects will utilize the
recently developed preparations of isolated gastric glands and
digitonin-permeabilized cells as well as the more traditional amphibian
gastric mucosa as in vitro models. A combination of modern biophysical,
biochemical, and morphological techniques will be used to investigate
selected aspects of cellular control mechanisms. The major areas to be
investigated include: 1) regulation of histamine H2-receptor; 2) the role
of protein kinases, specifically the cAMP-dependent and
calcium/phospholipid-dependent enzymes; 3) the basic mechanisms involved in
the morphological transition of the parietal cell which occurs with
stimulation; 4) the relationship between mitochondrial metabolism and
proton transport; and 5) the effects of omeprazole, an H-KATPase inhibitor,
on ion transport mechanisms. These areas are identified as important
regulatory sites, the investigation of which are timely and promises a high
rate of progress towards the longterm goal.
这个计划的长期目标是描述细胞的序列,
导致胃酸分泌的反应
粘膜,重点是那些反应,作为主要的
控制步骤。 最近对这一领域的兴趣源于其成功
抗分泌剂在溃疡病临床治疗中的应用
认识到质子运输在各种
生物过程。
目前的建议旨在扩大以前关于
细胞控制酸分泌,以便提供更详细的
了解控制机制。 项目将利用
最近开发的离体胃腺制剂,
毛地黄皂苷透化细胞以及更传统的两栖动物
胃粘膜作为体外模型。 结合现代生物物理学,
生物化学和形态学技术将用于研究
蜂窝控制机制的选定方面。 主要领域是
研究内容包括:1)组胺H2-受体的调节; 2)组胺H2-受体的作用
蛋白激酶,特别是cAMP依赖性和
钙/磷脂依赖酶; 3)参与的基本机制
壁细胞的形态转变,
刺激; 4)线粒体代谢与
质子转运;和5)奥美拉唑,一种H-KATP酶抑制剂,
离子传输机制 这些领域被确定为重要的
监管场所,调查及时,承诺高
实现长期目标的进度。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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