The dynamics of antimicrobial resistance genes in the pig and human gut microbiome in Uganda

乌干达猪和人类肠道微生物组抗菌素耐药性基因的动态

• 批准号: BB/P007767/1
• 负责人: Adrian Muwonge
• 金额: $ 38.85万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FP007767%2F1
• 关键词: dynamics; antimicrobial; resistance; genes; pig

The proposed research aims to gain fundamental insight into the generation, sharing and transfer of antimicrobial resistance (AMR) genes within and between humans and their pigs under natural settings that represent different levels of antibiotic use/misuse. Defining these dynamics offers the best chance of developing effective control measures and underwrites the efforts toward limiting irrational antibiotic use in both human and animal health care.This study combines the use of current molecular techniques, efficiency in data processing and a setting of epidemiological relevance to detect antibiotic driven changes in the gut bacteria. A forensic investigation of each of these changes would then reveal the fundamental characteristics of AMR genes in the gut and their transfer potential across the pig-human interface. The hypothesis to be tested is that people in contact with pigs in peri-urban settings are at higher risk of acquiring AMR genes across this interface than people in rural settings.The specific objectives are:1. To identify significant changes in taxonomic and AMR genes in gut microbiomes of people and their pigs in peri urban and rural settings over a one-year period using 16SrRNA, AMR gene probes and real time PCR. 2. To conduct forensic investigations of the significant changes detected in objective 1 using sequence based metagenomics on the corresponding gut microbiomes in parallel with whole genome sequencing and phenotypic AMR testing of sentinel bacteria to understand the underlying dynamics. 3. To predict, estimate and make inferences on AMR gene occurrence, taxa-linked within and between host exchange and their potential phenotypic effects using bioinformatic, statistical and support vector machine approaches on the data generated in 1&2.

这项研究旨在从根本上了解在自然环境下人类和猪之间抗菌素耐药性（AMR）基因的产生、共享和转移，这些自然环境代表了不同程度的抗生素使用/滥用。定义这些动态提供了最好的机会，制定有效的控制措施，并为限制人类和动物healthcare.This study的不合理的抗生素使用的努力提供了保证，结合使用当前的分子技术，数据处理的效率和流行病学的相关性，以检测抗生素驱动的肠道细菌的变化。对这些变化的法医调查将揭示肠道中AMR基因的基本特征及其在猪-人界面上的转移潜力。待检验的假设是，在城市周边环境中与猪接触的人比农村环境中的人在该界面上获得AMR基因的风险更高。利用16 SrRNA、AMR基因探针和真实的时间PCR技术，确定在一年的时间内，城市和农村环境中人及其猪的肠道微生物组中分类和AMR基因的显著变化。2.对目标1中检测到的显著变化进行法医调查，使用基于序列的宏基因组学对相应的肠道微生物组进行分析，同时进行哨兵细菌的全基因组测序和表型AMR检测，以了解潜在的动态。3.利用生物信息学、统计学和支持向量机方法对1&2中生成的数据进行预测、估计和推断AMR基因的发生、宿主内和宿主间的分类群关联及其潜在的表型效应。

项目成果

Molecular epidemiology of Mycobacterium bovis in Cameroon.

• DOI: 10.1038/s41598-017-04230-6
• 发表时间: 2017-07-05
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Egbe NF;Muwonge A;Ndip L;Kelly RF;Sander M;Tanya V;Ngwa VN;Handel IG;Novak A;Ngandalo R;Mazeri S;Morgan KL;Asuquo A;de C Bronsvoort BM
• 通讯作者: de C Bronsvoort BM

Identifying target areas for risk-based surveillance and control of transboundary animal diseases: a seasonal analysis of slaughter and live-trade cattle movements in Uganda.

• DOI: 10.1038/s41598-023-44518-4
• 发表时间: 2023-10-30
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Gonzalez-Gordon, Lina;Porphyre, Thibaud;Muwonge, Adrian;Nantima, Noelina;Ademun, Rose;Ochwo, Sylvester;Mwiine, Norbert Frank;Boden, Lisa;Muhanguzi, Dennis;Bronsvoort, Barend Mark de C.
• 通讯作者: Bronsvoort, Barend Mark de C.

Microbial characteristics of the DMFT index in a high HIV prevalence setting

• DOI: 10.21203/rs.3.rs-25139/v1
• 发表时间: 2020-05
• 作者: Dunstan Kalanzi;H. M. Kizza;D. Nakanjako;G. Mboowa;M. Mbabali;Edgar Kigozi;F. Ashaba;Ivan Sserwadda;D. Kateete;B. Acan;N. Sewankambo;A. Muwonge

Microbial characteristics of dental caries in HIV positive individuals.

• DOI: 10.3389/froh.2022.1004930
• 发表时间: 2022
• 期刊: FRONTIERS IN ORAL HEALTH
• 作者: Kalanzi, Dunstan;Mayanja-Kizza, Harriet;Nakanjako, Damalie;Semitala, Fred;Mboowa, Gerald;Mbabali, Muhammad;Kigozi, Edgar;Katabazi, Fred Ashaba;Sserwadda, Ivan;Kateete, David P;Achan, Beatrice;Sewankambo, Nelson K;Muwonge, Adrian
• 通讯作者: Muwonge, Adrian

Genetic Diversity of Cameroon Cattle and a Putative Genomic Map for Resistance to Bovine Tuberculosis.

• DOI: 10.3389/fgene.2020.550215
• 发表时间: 2020
• 期刊: Frontiers in genetics
• 影响因子: 3.7
• 作者: Callaby R;Kelly R;Mazeri S;Egbe F;Benedictus L;Clark E;Doeschl-Wilson A;Bronsvoort B;Salavati M;Muwonge A
• 通讯作者: Muwonge A