DEVELOPMENT OF CELL POLARIZATION IN EPITHELIAL CELLS

上皮细胞中细胞极化的发展

基本信息

批准号：
3228288
负责人：
CARLOS A RABITO
金额：
$ 15.18万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
1980
资助国家：
美国
起止时间：
1980-12-01 至 1990-06-30
项目状态：
已结题

项目摘要

The vectorial active transport of solutes and water across epithelial membranes results from the different morphological, biochemical and functional properties of the apical and basolateral aspects of the plasma cell membrane. Though cell polarization is implicit as the main premise in practically all the current models which have been proposed to explain the transepithelial active transport, the mechanisms involved in its subcellular and molecular development have not been elucidated. The aim of this research proposal is to understand the processes involved in the genesis and development of the epithelial cell polarization. The approach includes the use of cultured epithelial cell lines of renal origin that, like their "in vivo" counterpart, are able to perform transepthelial active transport. The development of epithelial cell polarization will be studied by monitoring different polarization markers during the conversion of a population of isolated cells into an epithelial membrane. In particular we intend to characterize the factors involved in the determination (signalling) of the polarization process by culturing the cells in interphases of different composition. We will determine which of the pathways proposed to explain the intracellular transport of membranes or macromolecules is or are involved in the assymetrical distribution of the membrane components. A combination of immunochemical, histochemical, and cell fractionation techniques will be used to approach this issue. Finally, we will analyze the mechanisms involved in the integration of polarization markers into the cell membrane. Different transport systems that become evident after their insertion in the cell membrane will be used in the analysis of this step.

溶质和水跨上皮的矢量积极运输膜来自不同的形态学，生化和等离子体顶端和基底外侧方面的功能特性细胞膜。尽管细胞极化是隐含的，作为主要前提实际上，所有当前的模型都提出了解释 transepithialial主动运输，其机制涉及尚未阐明亚细胞和分子发育。目的该研究建议是了解上皮细胞极化的起源和发育。方法包括使用肾脏起源的培养的上皮细胞系，就像他们的“体内”对应物一样运输。将研究上皮细胞极化的发展通过监视转化过程中不同的极化标记分离的细胞种群进入上皮膜。特别是我们打算表征确定的因素极化过程的（信号）通过培养细胞不同组成的相互重点。我们将确定哪个建议解释膜或大分子是或参与的膜组件。免疫化学，组织化学和细胞分馏技术将用于解决此问题。最后，我们将分析集成的机制极化标记进入细胞膜。不同的运输系统将使用在细胞膜中插入后很明显在分析此步骤中。