REAL-TIME FLUORESCENCE-ACTIVATED RENAL MONITOR

实时荧光激活肾脏监测仪

• 批准号: 2874283
• 负责人: CARLOS A RABITO
• 金额: $ 14.07万
• 依托单位: UNION BIOMETRICA, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2874283
• 关键词: acute renal failure; biomedical equipment development; chemical synthesis; fluorescent dye /probe; glomerular filtration rate; laboratory rat; patient monitoring device

DESCRIPTION (adapted from the applicant's abstract): Real-time and accurate data of the renal function are needed to develop new approaches for the prevention and treatment of acute renal failure (ARF). Current techniques have neither the accuracy nor the resolution time required. A highly innovative approach, was recently developed for the real-time and accurate measurement of glomerular filtration rate (GFR). The technique determines, minute-to-minute, the rate of disappearance from the extracellular space (ES) of a radiolabeled GFR agent as measured with an external radioactivity counting device. Although the approach showed excellent results, the use of radioactivity, limit its application. As an alternative, this project proposes the use a GFR agent labeled with a laser fluorescent dye (FGFR). The project involves the synthesis of the agent, the design and assembly of an instrument to measure the laser-induced tissue fluorescence (LITF) and the initial validation of the technique in rats. The synthesis of the agent will be performed by labeling with a laser fluorescent dye a well established GFR agent. A laser photodiode will be used in the instrument for the excitation beam. The LITF will be measured and recorded for further processing. At a constant excitation, LITF should be proportional to the tissue concentration of FGFR. This concept will be validated in rats by comparing the LITF with direct tissue concentration after extraction of the dye. This alternative method should represent a significant advance, not only for immediate detection of renal dysfunction to avert ARF, but also for the instant evaluation of the response to therapy in patients with ARF. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（改编自申请人的摘要）：实时准确 需要肾功能的数据来开发新方法 急性肾衰竭（ARF）的预防和治疗。当前的技术具有 不需要准确性和分辨率时间。高度创新的 最近开发了用于实时和准确测量的方法 肾小球滤过率（GFR）。该技术决定了分钟至分钟 放射性标记的细胞外空间（ES）消失率 用外部放射性计数设备测量的GFR代理。 尽管该方法显示出良好的结果，但放射性的使用，极限 它的应用。作为替代方案，该项目建议使用GFR代理 用激光荧光染料（FGFR）标记。该项目涉及合成 代理，仪器的设计和组装以测量 激光诱导的组织荧光（LITF）和初始验证 老鼠的技术。代理的合成将通过标记执行 用激光荧光染料染料良好的GFR剂。激光光电二极管 将在仪器中用于激发光束。 litf将是 测量并记录以进行进一步处理。持续激发，litf 应与FGFR的组织浓度成正比。这个概念会 通过将LITF与直接组织浓度进行比较，可以在大鼠中验证 提取染料后。这种替代方法应代表 重大进展，不仅用于立即检测到肾功能障碍 避免ARF，但也可以立即评估对治疗的反应 ARF患者。 拟议的商业应用程序：不可用