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Tapping the Unused Potential of Photosynthesis

挖掘光合作用未利用的潜力

基本信息

批准号：
BB/P019331/1
负责人：
Thomas Bibby
金额：
$ 60.3万
依托单位：
University of Southampton
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FP019331%2F1
关键词：
Tapping Unused Potential Photosynthesis

项目摘要

Photosynthesis is the pivotal biological reaction on the planet, providing the food we eat and the oxygen we breathe, and removing CO2 from the atmosphere. Photosynthesis consists of two reactions: the light reactions absorb light energy from the sun and use this to split water (H2O) into electrons, protons and oxygen, and the dark reactions use the electrons and protons from the light reactions to 'fix' CO2 from the atmosphere into simple sugars that are the basis of the food chain. Importantly, the light reactions have a much higher capacity than the dark reactions, resulting in much of the absorbed light energy being dissipated rather than being used to 'fix' CO2.In this proposal we will use synthetic-biology methods to engineer an additional enzyme (a cytochrome P450, CYP) in-between the light reactions and the dark reactions. This will 'rewire' photosynthesis such that more absorbed light is used to power useful chemical reactions. This work therefore represents an innovation whereby a range of additional valuable chemical reactions can be powered by the sun in cyanobacteria and plants. We have previously developed a 'platform' cyanobacterial cell line where the 'wasted' electrons of photosynthesis are rewired (using a CYP) to degrade the pollutant atrazine (a herbicide used in agriculture). Atrazine while banned in the EU is still one of the most prevalent pesticides in some groundwater systems. This cell line may be used in the efficient bioremediation of such polluted wastewater areas.In this proposal we aim to further develop the use of CYP in rewiring photosynthesis such that we can improve and regulate the yield of the desired products, as well as enhance the diversity of products that can be powered by light. Further, we will fully characterise the physiology of engineered cell lines such that we can interrogate the fundamental mechanism of this photosynthetic electron transfer. In addition, we aim to transfer this technology to the model higher plant Arabidopsis as a first step in improving photosynthetic potential in crop species.The 'added value' we aim to introduce into cyanobacteria and plants may be a critical step toward the commercial realisation of using photosynthetic species to generate 'biofuels' that may one day replace our current dependence on fossil fuels.

光合作用是地球上关键的生物反应，提供我们吃的食物和我们呼吸的氧气，并从大气中去除二氧化碳。光合作用由两个反应组成：光反应从太阳吸收光能，并利用其将水（H2O）分解为电子，质子和氧气，暗反应使用光反应的电子和质子将大气中的CO2“固定”为单糖，这是食物链的基础。重要的是，光反应比暗反应具有更高的能力，导致大部分吸收的光能被耗散，而不是用于“固定”CO2。在本提案中，我们将使用合成生物学方法在光反应和暗反应之间设计一种额外的酶（细胞色素P450，CYP1A1）。这将“重新布线”光合作用，使更多的吸收光用于推动有用的化学反应。因此，这项工作代表了一项创新，即一系列额外的有价值的化学反应可以由太阳在蓝藻和植物中提供动力。我们之前已经开发了一种“平台”蓝藻细胞系，其中光合作用的“浪费”电子被重新连接（使用一种催化剂）以降解污染物阿特拉津（农业中使用的除草剂）。阿特拉津虽然在欧盟被禁止，但仍然是一些地下水系统中最普遍的农药之一。该细胞系可用于此类污染废水区域的有效生物修复。在本提案中，我们的目标是进一步开发在重新布线光合作用中的使用，以便我们可以提高和调节所需产物的产量，以及增强可由光驱动的产物的多样性。此外，我们将充分研究工程细胞系的生理学，以便我们可以询问这种光合电子转移的基本机制。此外，我们的目标是将这项技术转移到模式高等植物拟南芥中，作为提高作物物种光合潜力的第一步。我们旨在引入蓝藻和植物的“附加值”可能是实现商业化的关键一步，利用光合物种产生“生物燃料”，有朝一日可能会取代我们目前对化石燃料的依赖。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Directing cyanobacterial photosynthesis in a cytochrome c oxidase mutant using a heterologous electron sink.

DOI：
10.1093/plphys/kiac203
发表时间：
2022-08-01
期刊：
PLANT PHYSIOLOGY
影响因子：
7.4
作者：
Torrado, Alejandro;Connabeer, Hannah M.;Rottig, Annika;Pratt, Nicola;Baylay, Alison J.;Terry, Matthew J.;Moore, C. Mark;Bibby, Thomas S.
通讯作者：
Bibby, Thomas S.

An inducible expression system in the alga Nannochloropsis gaditana controlled by the nitrate reductase promoter

DOI：
10.1007/s10811-018-1510-6
发表时间：
2019-02-01
期刊：
JOURNAL OF APPLIED PHYCOLOGY
影响因子：
3.3
作者：
Jackson, Harry O.;Berepiki, Adokiye;Bibby, Thomas S.
通讯作者：
Bibby, Thomas S.

Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria.

DOI：
10.1073/pnas.2021523118
发表时间：
2021-03-16
期刊：
Proceedings of the National Academy of Sciences of the United States of America
影响因子：
11.1
作者：
Santos-Merino M;Torrado A;Davis GA;Röttig A;Bibby TS;Kramer DM;Ducat DC
通讯作者：
Ducat DC

Isolation and molecular characterisation of Dunaliella tertiolecta with truncated light-harvesting antenna for enhanced photosynthetic efficiency