ELECTRON PROBE ANALYSIS OF RENAL CONCENTRATION MECHANISM

肾脏浓缩机制的电子探针分析

• 批准号: 3233257
• 负责人: CLAUDE P LECHENE
• 金额: $ 9.13万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3233257
• 关键词: acidity /alkalinity; diuresis; electron probe spectrometry; kidney function; laboratory rabbit; laboratory rat; membrane transport proteins; micropuncture; renal medulla; renal tubular transport; renal tubule; secretion; sorbitol; urea

The goal of this project is to better understand the renal concentrating mechanism. Electron probe analysis of frozen hydrated bulk kidneys will allow us to measure the renal concentration gradient of Na, K and Cl directly along the longitudinal cortico-papillary axis. Analysis of frozen hydrated bulk kidneys will provide the composition of tubular fluid in outer and inner medullary segments of the nephron, inaccessible to sampling by micropuncture. Microfluorescence urea determination of renal thin frozen slices will provide the cortico-papillary urea gradient at high resolution. We will attempt measuring the gradient profile of sorbitol accumulation in antidiuresis. Analysis will be performed in three types of mammals: rats, rabbits and psammomys, each of which may have a different renal concentrating mechanism. Analysis will performed in extreme diuretic states: Water deprivation, osmotic diuresis, and, in the rat, water diuresis by water load. Understanding of the renal concentrating mechanism is stalled by the lack of new data or by the reliance on old data which do not provide enough resolution. With gradient profile and composition of intratubular fluid in the outer and inner medulla, we will be able to evaluate ours and other models of renal concentration in the inner medulla.

本项目的目的是为了更好地了解肾浓缩。 机制。冷冻水合大块肾组织的电子探针分析 允许我们测量肾脏中钠、钾和氯的浓度梯度 直接沿着皮质乳头状轴的纵轴。对冻结物的分析 水合的大块肾脏将提供肾小管液体的成分 肾单位的外、内髓节段，不能取材 通过显微穿刺法。微量荧光尿素测定肾脏薄层 冷冻切片将提供高水平的皮质-乳头状尿素梯度 决议。我们将尝试测量山梨醇的梯度分布 积聚在抗利尿中。分析将在三种类型的 哺乳动物：老鼠、兔子和沙鼠，每种动物都可能有不同的 肾浓缩机制。分析将在极端利尿剂中进行 状态：缺水，渗透性利尿，在大鼠体内，水 水负荷利尿。对肾浓缩机制的认识 由于缺乏新数据或依赖旧数据而停滞不前 没有提供足够的分辨率。具有渐变轮廓和组成 在外髓和内髓的管内液体，我们将能够 评估我们的和其他模型的肾内髓质中的肾脏浓度。