Regulation of diuresis by 2 types of antidiuretic hormone receptor in chronic renal failure and therapeutic investigation of edema

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We first investigated urinary excretion of aquaporin 2 (AQP2) using Western blot analysis. However, AQP2 was not detectable in the urine. We have been still trying to detect urinary excretion of AQP2 by increasing the sensitivity of Western blot analysis. Urinary excretion of AQP2 was also examined in rats with metabolic acidosis. Urinary excretion of AQP2 was significantly decreased in metabolic acidosis. However, the expressions of AQP2 mRNA and protein in outer medullary collecting ducts were increased. Differential centrifugation revealed that AQP2 expression was decreased in membrane-rich fraction but increased in intracellular vesicle-rich fraction. These data suggest that trafficking of AQP2 from intracellular vesicles to apical membrane is inhibited in metabolic acidosis. The precise mechanisms of the disturbance has to be examined further.