STUDY OF A LOW-POTENTIAL ELECTROCHEMICAL GLUCOSE SENSOR

目标是开发一种电化学葡萄糖传感器，它是 能够被植入血管或细胞外空间 糖尿病患者连续活体血糖监测 水平。它的使用将消除间歇性的不便 抽血进行血糖测定，以及控制滞后 这是强加的。直接的床边读数将有利于急性脑血管意外 糖尿病和这样的传感器，当与胰岛素输液泵结合时，以及 与反馈系统相连接，将完成一个 完整的人工内分泌胰腺。一些泵和反馈 该系统目前正在美国进行临床评估。 人工胰腺的中心是葡萄糖传感器，它远远 在它的发展中落后于其他组件。提供这样的一种 传感器将成为临床实践和基础研究的突破 糖尿病及其相关并发症的调查。 电位动力学研究揭示了两种新的不同的低电势氧化还原 可用于葡萄糖的光滑铂电极上的峰 甚至在人血清的超滤液中也进行监测。这种反应是 由于电极表面是通过电化学再生的，因此可重现性 脉搏跳动。两个峰位于定义明确的电位：-0.72V -0.80V(阴极还原)和-0.80V(阴极还原)。这些 电势是稳定的，不会随着葡萄糖的增加而移动 集中精神。体外研究表明，使用这种材料的电极 反应应在葡萄糖水平达400毫克/分升时可操作。 具体目标是：(1)构建一个标准的、可控的几何图形 使用厚膜沉积技术测试电极；(20使用此方法 电极在参数检验中的作用，以表征 产生氧化还原峰；(3)选择合适的伏安条件 用于传感器的实际开发；(4)对传感器的性能进行评估 潜在存在时葡萄糖峰的特异性 可能存在于人体血液和组织中的干扰物质 液体；(5)确定是否有微量的PAT和Ag在 延长伏安法；(6)设计和制造一种小型化、 采用薄膜沉积技术的植入式电极阵列。

The goal is the development of an electrochemical glucose sensor which is capable of being implanted into the vascular or extracellular space of a diabetic patient for continuous in vivo monitoring of his blood sugar level. Its use would eliminate the inconvenience of intermittent withdrawal of blood for glucose assay, as well as the control lag which this imposes. A direct bedside readout would benefit the acute case of diabetes and such a sensor, when coupled with an insulin infusion pump, and interfaced with a feedback system, would complete the development of an integrated artificial endocrine pancreas. A number of pumps and feedback systems are currently under clinical evaluation in the United States. Central to the artificial pancreas is the glucose sensor which is far behind the other components in its development. The availability of such a sensor would constitute a breakthrough for both clinical practice and basic investigation of diabetes and its associated complications. Potentiodynamic studies have revealed two new distinct low-potential redox peaks at the smooth Pt electrode that can be utilized for glucose monitoring even in the ultrafiltrate of human serum. This reaction is reproducible since the electrode surface is regenerated by electrochemical pulsing. The two peaks are located at well-defined potentials: -0.72 V (anodic oxidation) and -0.80 V (cathodic reduction) vs Ag/AgCl. These potentials are stable and do not shift with increasing glucose concentration. The in vitro study has shown that an electrode using this reaction should be operable at glucose levels up to 400 mg/dl. The specific aims are: (1) to construct a standard, controlled geometry test electrode using thick film deposition technique; (20 to use this electrode in parametric tests to characterize the mechanism by which the redox peaks are generated; (3) to select the proper voltammetric conditions for the practical development of the sensor; (4) to evaluate the specificity of the peaks for glucose in the presence of potentially interfering substances which might be present in human blood and tissue fluid; (5) to determine if trace amounts of Pat and Ag are released during prolonged voltammetry; (6) to design and construct a miniaturized, implantable electrode array using thin film deposition techniques.