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STUDY OF A LOW-POTENTIAL ELECTROCHEMICAL GLUCOSE SENSOR

低电位电化学葡萄糖传感器的研究

基本信息

批准号：
3152582
负责人：
SHANG J YAO
金额：
$ 9.27万
依托单位：
MONTEFIORE UNIVERSITY HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-12-01 至 1985-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3152582
关键词：
artificial endocrine pancreas atomic absorption spectrometry blood chemistry blood glucose clinical biomedical equipment electrical potential electrochemistry gas chromatography human subject human tissue insulin silver chloride electrode urinalysis

项目摘要

The goal of the proposed project is the development of an electrochemical glucose sensor which is capable of being implanted into the vascular of extracellular space of a diabetic patient for continuous in vivo monitoring of his blood sugar level. The use of this sensor would eliminate the intermittent withdrawal of blood for glucose assay. Such a sensor, when coupled with an insulin infusion pump and interfaced with a feedback system, would complete the development of an integrated artificial endocrine pancreas. A number of pumps and feedback systems are currently under clinical evaluation in the United States. Central to the artificial pancreas is the glucose sensor which is far behind the other components in its development. The availability of such a sensor would constitute a breakthrough for both clinical practice and basic investigation of diabetes and its associated complications. Potentiodynamic studies in this laboratory have revealed two new distinct low-potential anodic peaks at the smooth Pt electrode that can be utilized for glucose monitoring even in the presence of human serum. This reaction is reproducible since the electrode surface is regenerated by cyclic electrochemical pulsing. The two peaks are located at well defined potentials -0.58 V and -0.43 V vs Ag/AgC1. These potentials are stable and do not shift with increasing glucose concentration. The in vitro study has shown that an electrode using this reaction should be operable at glucose levels up to 400 mg/dl. The specific objectives of the proposed three-year pilot study are: (1) to clearly characterize the mechanism by which these two anodic peaks are generated; (2) to select the proper cyclic voltammetric conditions for the practical development of the sensor; (3) to evaluate the specificity of the anodic peaks for glucose in the presence of potentially interfering substances which might be present in human blood and drugs; (4) to determine if trace amounts of Pt are released during prolonged cyclic voltammetry (possible toxicity); and (5) to fabricate a prototype sensor to be integrated into an existing insulin infusion pump and feedback artificial pancreas system.

该项目的目标是开发一种电化学葡萄糖传感器，其能够被植入血管中，用于连续体内监测的糖尿病患者的细胞外空间他的血糖水平。该传感器的使用将消除间歇抽取血液进行葡萄糖测定。这种传感器，当其与胰岛素输注泵耦合并与反馈接口系统，将完成一个集成的人工开发内分泌胰腺许多泵和反馈系统目前被在美国进行临床评估。人工智能的核心胰腺是葡萄糖传感器，它远远落后于其他组件，它的发展。这种传感器的可用性将构成糖尿病临床实践和基础研究的突破及其相关并发症。本实验室的动电位研究揭示了两种新的不同的在光滑的Pt电极上的低电位阳极峰，用于即使在人血清存在下的葡萄糖监测。该反应由于电极表面通过循环再生，因此具有可重复性电化学脉冲这两个峰位于定义明确的电位为-0.58 V和-0.43 V，相对于Ag/AgCl。这些电位稳定，不随葡萄糖浓度的增加而变化。体外研究已经表明使用该反应的电极应该在葡萄糖水平高达400 mg/dl。这项为期三年的试验计划的具体目标如下：（一）清楚地描述了这两个阳极峰的机制，（2）选择合适的循环伏安法条件，传感器的实际开发;（3）评估传感器的特异性。存在潜在干扰时葡萄糖的阳极峰可能存在于人体血液和药物中的物质;（4）确定在长时间循环期间是否释放痕量Pt 伏安法（可能的毒性）;和（5）制造原型传感器，可以集成到现有的胰岛素输注泵中人工胰腺系统