PERIPHERAL AND CENTRAL SATIETY ACTIONS OF BOMBESIN

这个项目的目标是明确内源性的作用 哺乳动物外周血中蛙毒素样多肽 (胃肠)产生和中枢神经处理 用于(A)限制进食的生理饱腹感信号 大小和(B)确定餐后中间餐的长度 大鼠的时间间隔。 实现这一目标的实验步骤顺序如下 1)确定外源物质的作用部位， 外周给药蛙毒素样多肽 限制进餐数量，延长餐后间隔时间； (2)确定这些神经系统的中枢神经系统通路 两种效应；(3)确定外周、内源性BBS样 这两种蛋白的完整表达都需要多肽。 效果；(4)确定脑内微量注射 外源BBS样肽产生行为特异性饱足感 对进餐大小和餐后间隔的影响； (5)确定BBS样多肽是否在这些大脑中的作用 外源的两种饱腹感是必需的， 外周给药的BBS样肽；(6)确定 两种内源性BBS样肽在这些脑部位的作用是 对于进食食物的两种饱腹感来说是必要的，在饭菜上 体型和餐后间隔。 如果蛙皮蛋白家族的多肽被证明扮演着 周围性餐后饱腹感的生理机制 和/或中心站点，这一结果将从根本上 对正常进食行为的分析和对 两者的病理生理测定及治疗 人类的主要饱腹症，神经性暴食症和肥胖症。

The goal of this project is to specify the roles of endogenous mammalian bombesin-like peptides in the peripheral (gastrointestinal) generation and in the central neural processing of the physiological satiety signals which serve (a) to limit meal size and (b) to determine the length of the postprandial intermeal interval in the rat. The sequence of experimental steps to achieve this goal will be as follows: 1) Determine the sites of action of exogenous, peripherally-administered bombesin (BBS)-like peptides when they limit meal size and prolong the postprandial intermeal interval; (2) Determine the pathways to the central nervous system for these two effects; (3) Determine if peripheral, endogenous BBS-like peptides are necessary for the complete expression of these two effects; (4) Determine if intracerebral microinjections of exogenous BBS-like peptides produce behaviorally specific satiety effects on meal size and on the postprandial intermeal interval; (5) Determine if the action of BBS-like peptides at these brain sites is necessary for the two satiety effects of exogenous, peripherally-administered BBS-like peptides; (6) Determine if the action of two-endogenous BBS-like peptides at these brain sites is necessary for the two satiety effects of ingested food, on meal size and on the postprandial intermeal interval. If the bombesin family of peptides is shown to play roles as physiological mechanisms of postprandial satiety at peripheral and/or central sites, this result will have fundamental implications for the analysis of normal feeding behavior and for the determination of the pathophysiology and treatment of the two major satiety disorders in humans, bulimia nervosa and obesity.