PERIPHERAL AND CENTRAL SATIETY ACTIONS OF BOMBESIN

本项目的目标是明确内源性 哺乳动物外周血蛙皮素样肽 （胃肠道）生成和中枢神经处理 生理饱足信号的作用（a）限制进餐 大小和（B）确定餐后间隙的长度 在大鼠中的间隔。 实现这一目标的实验步骤顺序如下： 具体如下：1）确定外源性， 外周给药的蛙皮素（BBS）样肽， 限制进餐量，延长餐后间隔时间; (2)确定中枢神经系统的通路， 两种效应;（3）确定外周、内源性BBS样 肽是这两种蛋白完全表达所必需的。 （4）确定脑内微量注射 外源性BBS样肽产生行为特异性饱腹感 对膳食量和餐后餐间间隔的影响; (5)确定BBS样肽在这些大脑中的作用是否 两个饱腹感的作用部位是必需的外源性， 外周施用的BBS样肽;（6）确定是否存在 两种内源性BBS样肽在这些脑部位的作用是 必要的两个饱腹感的影响，摄入的食物，对膳食 大小和餐后间隔。 如果蛙皮素家族的肽被证明发挥作用， 餐后饱腹感的外周生理机制 和/或中心站点，该结果将具有基本的 对分析正常摄食行为和 病理生理学的确定和两种治疗 人类的主要饱腹感障碍，神经性贪食症和肥胖症。

The goal of this project is to specify the roles of endogenous mammalian bombesin-like peptides in the peripheral (gastrointestinal) generation and in the central neural processing of the physiological satiety signals which serve (a) to limit meal size and (b) to determine the length of the postprandial intermeal interval in the rat. The sequence of experimental steps to achieve this goal will be as follows: 1) Determine the sites of action of exogenous, peripherally-administered bombesin (BBS)-like peptides when they limit meal size and prolong the postprandial intermeal interval; (2) Determine the pathways to the central nervous system for these two effects; (3) Determine if peripheral, endogenous BBS-like peptides are necessary for the complete expression of these two effects; (4) Determine if intracerebral microinjections of exogenous BBS-like peptides produce behaviorally specific satiety effects on meal size and on the postprandial intermeal interval; (5) Determine if the action of BBS-like peptides at these brain sites is necessary for the two satiety effects of exogenous, peripherally-administered BBS-like peptides; (6) Determine if the action of two-endogenous BBS-like peptides at these brain sites is necessary for the two satiety effects of ingested food, on meal size and on the postprandial intermeal interval. If the bombesin family of peptides is shown to play roles as physiological mechanisms of postprandial satiety at peripheral and/or central sites, this result will have fundamental implications for the analysis of normal feeding behavior and for the determination of the pathophysiology and treatment of the two major satiety disorders in humans, bulimia nervosa and obesity.