THE ADIPOSE CONVERSION OF 3T3 CELLS

当前脂肪3T3细胞停止生长并与外部 成脂因子，它们开始转化为脂肪细胞。这是一个 脂肪细胞发育早期形成的忠实模型。在……里面 脂肪转化的过程中有一个广泛的修改 细胞质的蛋白质组成；这是必要的，以提供 与生产性能有关的生脂和脂解酶及辅助蛋白 细胞类型的分化功能。使用cdna克隆和 最终，一些参与基因克隆的蛋白质的基因组克隆 为了实现差异化，我们将研究 脂肪转化，以了解细胞如何协调 这组基因的表达。转录和基因转录的研究 在前脂肪3T3细胞、转化的3T3脂肪细胞和在 另一株3T3细胞不能进行转换 成脂因子将使我们能够定义基因的顺序 请回答。特别是，我们将对这些事件进行动力学分析 在脂肪转化的过程中。的顺序之间的关系 这些基因的表达随着染色质结构的变化而变化 探索过了。这些基因的表达将在其他组织中进行研究， 例如肝脏和大脑，它们的分化程序与 程序中脂肪分化。通过这种方式，我们希望了解到 给定基因的表达可以集成到不同的程序中 不同的细胞类型。 这些研究为肥胖症的研究提供了一种途径。 细胞。对分子和细胞变化的分析是必要的 由其前体形成的脂肪细胞可能会脱落 关于增生性肥胖症的起源。

When preadipose 3T3 cells arrest their growth and interact with an external adipogenic factor, they begin to convert to adipose cells. This is a faithful model of the formation of adipose cells in early developement. In the process of adipose conversion there is an extensive revision of the protein composition of the cytoplasm; this is necessary in order to provide the lipogenic and lipolytic enzymes and accesssory proteins for performance of the differentiated functions of the cell type. Using cDNA clones and ultimately genomic clones for some of the proteins participating in the differentiation, we will study the integrated or programmatic aspects of the adipose conversion, in order to learn how the cell coordinates the expression of this group of genes. Studies of transcription and mRNA formation in preadipose 3T3 cells, in converted 3T3 adipose cells and in cells of another 3T3 line unable to undergo conversion in response to adipogenic factor will enable us to define the order in which the genes respond. In particular, we will perform a kinetic analysis of these events during the course of adipose conversion. The relation of the order of expression of these genes to changes in chromatin structure will be explored. The expression of these genes will be studied in other tissues, such as liver and brain, whose program of differentiation overlaps the program in adipose differentiation. In this way we hope to learn how the expression of a given gene can be integrated into different programs in different cell types. These studies permit an approach to the study of obesity using cultured cells. Analysis of the molecular and cellular changes necessary for the formation of adipose cells from their precursors may be expected to shed light on the genesis of hyperplastic obesity.