NEAR ULTRAVIOLET RAMAN STUDIES OF CYTOCHROME P450
细胞色素 P450 的近紫外拉曼研究
基本信息
- 批准号:3233332
- 负责人:
- 金额:$ 12.62万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1984
- 资助国家:美国
- 起止时间:1984-06-01 至 1988-11-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have observed detailed and informative resonance Raman spectra from the
heme proteins cytochrome P450 and cytochrome c using near ultraviolet laser
excitation. We propose to extend our measurements into the visible using
our tunable dye laser and further into the ultraviolet using harmonic
generation and optical multichannel analyzer (OMA) detection. We plan to
specifically focus on Raman spectral studies of important catalytic
intermediate states such as the reduced and oxygenated P450 complexes in
order to help deduce mechanisms involved in oxygen binding and cleavage in
this important class of metabolic enzymes. The long term objective is to
use resonance Raman scattering to gain new information about the active
site structure and function of cytochrome P450 and other heme proteins and
to achieve a better understanding of metalloporphyrin optical absorption
spectra. Photosensitive intermediate states will be studied using OMA
detection and our dual arm Raman difference spectrometer will be utilized
in a variety of experiments involving cryogenic temperatures, isotopic
labels, pH and pressure perturbations, etc. The theoretical implications
of 3-body normal mode oscillator models will be further pursued and the
"transform" based theories relating absorption and Raman excitation profile
measurements will be applied to heme protein systems such as cytochrome c,
hemoglobin and cytochrome P450. Fluorescence excitation profile
measurements of heme systems will be made using our newly developed
technique for absolute quantum yield determination. Photoacoustic
spectroscopy will be used to aid in our understanding of these
fluoresecence experiments and the accompanying rapid nonradiative
relaxation channels present in heme protein systems.
我们观察到了详细的和信息量丰富的共振拉曼光谱
近紫外光激光作用下的细胞色素P450和细胞色素C
激动人心。我们建议将我们的测量扩展到可见光,使用
我们的可调谐染料激光器,进一步利用谐波进入紫外光
产生和光学多道分析器(OMA)检测。我们计划
重点研究了重要催化剂的拉曼光谱
中间态,如还原和氧化的P450络合物
以帮助推断参与氧结合和裂解的机制
这类重要的代谢酶。长期目标是
利用共振拉曼散射获得有关活性物质的新信息
细胞色素P450和其他血红素蛋白的位置结构和功能
为了更好地理解金属卟啉的光吸收
光谱。光敏中间态将使用OMA进行研究
检测和我们的双臂拉曼差分光谱仪将使用
在涉及低温的各种实验中,同位素
标签、pH和压力扰动等。理论含义
将进一步研究三体简正振荡器模型,并
基于“变换”理论的吸收和拉曼激发谱
测量将应用于血红素蛋白系统,如细胞色素C,
血红蛋白和细胞色素P450。荧光激发谱
血红素系统的测量将使用我们新开发的
绝对量子产额测定技术。光声
光谱学将用来帮助我们理解这些
荧光实验和伴随的快速非辐射
在血红素蛋白系统中存在松弛通道。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Paul M. Champion其他文献
Squeezing Proteins at the Unfolding Limit
- DOI:
10.1016/j.bpj.2019.11.378 - 发表时间:
2020-02-07 - 期刊:
- 影响因子:
- 作者:
Prabhat Tripathi;Abdelkrim Bennabbas;Paul M. Champion;Meni Wanunu - 通讯作者:
Meni Wanunu
Low frequency dynamics of Cystathionine beta-synthase
- DOI:
10.1016/j.bpj.2008.12.2229 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Karunakaran Venugopal;Yuhan Sun;Zhenyu Zhang;Abdelkrim Benabbas;Sangita Singh;Ruma Banerjee;Paul M. Champion - 通讯作者:
Paul M. Champion
Reactive Vibrational Dynamics of Iron in Heme
- DOI:
10.1016/j.bpj.2008.12.2228 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Alexander Barabanschikov;J. Timothy Sage;W. Robert Scheidt;Chuanjiang Hu;Minoru Kubo;Paul M. Champion;Jiyong Zhao;Wolfgang Sturhahn;E. Ercan Alp - 通讯作者:
E. Ercan Alp
Single Protein Trapping on Ultrathin Asymmetric Solid-State Nanopores
- DOI:
10.1016/j.bpj.2019.11.970 - 发表时间:
2020-02-07 - 期刊:
- 影响因子:
- 作者:
Hirohito Yamazaki;Fanjun Li;Abdelkrim Benabbas;Benjamin Cressiot;Paul M. Champion;Min Chen;Meni Wanunu - 通讯作者:
Meni Wanunu
Investigation of the Low Frequency Dynamics of Heme Proteins: Native and Mutant Cytochromes P450<sub>cam</sub> and Redox Partner Complexes
- DOI:
10.1016/j.bpj.2009.12.3512 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:
- 作者:
Karunakaran Venugopal;Ilia Denisov;Aditi Das;Stephen G. Sligar;Paul M. Champion - 通讯作者:
Paul M. Champion
Paul M. Champion的其他文献
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{{ truncateString('Paul M. Champion', 18)}}的其他基金
Femtosecond Coherence Spectroscopy and Ultrafast Kinetic Investigations of Heme P
血红素 P 的飞秒相干光谱和超快动力学研究
- 批准号:
8000136 - 财政年份:2010
- 资助金额:
$ 12.62万 - 项目类别:
CARS Imaging for Studies of Cell Metabolism
用于细胞代谢研究的 CARS 成像
- 批准号:
6445114 - 财政年份:2002
- 资助金额:
$ 12.62万 - 项目类别:
NEAR-ULTRAVIOLET RAMAN STUDIES OF CYTOCHROME P450
细胞色素 P450 的近紫外拉曼研究
- 批准号:
2139477 - 财政年份:1984
- 资助金额:
$ 12.62万 - 项目类别:
NEAR ULTRAVIOLET RAMAN STUDIES OF CYTOCHROME P450
细胞色素 P450 的近紫外拉曼研究
- 批准号:
3153664 - 财政年份:1984
- 资助金额:
$ 12.62万 - 项目类别:
NEAR ULTRAVIOLET RAMAN STUDIES OF CYTOCHROME P450
细胞色素 P450 的近紫外拉曼研究
- 批准号:
6329326 - 财政年份:1984
- 资助金额:
$ 12.62万 - 项目类别:
NEAR ULTRAVIOLET RAMAN STUDIES OF CYTOCHROME P450
细胞色素 P450 的近紫外拉曼研究
- 批准号:
6124873 - 财政年份:1984
- 资助金额:
$ 12.62万 - 项目类别:
NEAR ULTRAVIOLET RAMAN STUDIES OF CYTOCHROME P450
细胞色素 P450 的近紫外拉曼研究
- 批准号:
3233335 - 财政年份:1984
- 资助金额:
$ 12.62万 - 项目类别:
NEAR ULTRAVIOLET RAMAN STUDIES OF CYTOCHROME P450
细胞色素 P450 的近紫外拉曼研究
- 批准号:
3233334 - 财政年份:1984
- 资助金额:
$ 12.62万 - 项目类别:
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