Creating a spatially defined, multidimensional, protein interactome of the eukaryotic algal CO2 concentrating mechanism

创建真核藻类 CO2 浓缩机制的空间定义、多维蛋白质相互作用组

基本信息

批准号：
BB/R001014/1
负责人：
Luke Mackinder
金额：
$ 68.18万
依托单位：
University of York
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR001014%2F1
关键词：
Creating spatially defined multidimensional protein

项目摘要

Photosynthesis harnesses energy from the sun to fix carbon dioxide (CO2) into sugars and the protein building blocks of life. To enhance photosynthesis some plants and nearly all algae have evolved mechanisms to increase the accumulation of CO2 from their surrounding environment, this process in known as a CO2 concentrating mechanism (CCM). It is predicted that the transfer of a CCM to crop plants, such as rice and wheat that have failed to evolve CCMs, could increase yields by up to 60%. A promising CCM donor candidate is a green alga called Chlamydomonas, which has a highly efficient CCM. For the successful implementation of an algal CCM into crop plants it is essential that all the components are known, where they are found in the cell and how they function together. This project aims to rapidly identify all the proteins that make up the Chlamydomonas CCM and to determine how they interact with each other to form a functional unit that enhances CO2 uptake. To achieve this, we will determine where the different protein components of the CCM are located in the algal cell, we will then identify what proteins they are interacting with and how these interactions change when the CCM is switched on and off. This will allow us to understand the network of the CCM and how it is regulated. Finally, using mutants that lack individual proteins of the CCM, we will determine the functional importance of each protein component. The localisation, interaction and protein function data will be combined to create a detailed 3D map of the CCM that can be easily explored through an open-access, online interactive viewing platform. It is anticipated that these data will facilitate the transfer of a CCM into crop plants to increase photosynthesis and yields.

光合作用利用来自太阳的能量，将二氧化碳（CO2）固定到糖和生命的蛋白质基础中。为了增强光合作用，某些植物和几乎所有藻类都具有从周围环境中增加二氧化碳积累的机制，这种过程以二氧化碳浓缩机制（CCM）而被称为。可以预测，将CCM转移到未能进化CCM的水稻和小麦等农作物植物中，可能会将产量提高高达60％。有希望的CCM供体候选者是一种称为Chlamydomonas的绿藻，其效率高度有效。为了成功地实施藻类CCM到作物植物中，必须将所有组件都知道，在细胞中发现它们以及它们如何共同发挥作用。该项目旨在快速识别构成衣原体CCM的所有蛋白质，并确定它们如何相互相互作用，以形成增强CO2摄取的功能单元。为了实现这一目标，我们将确定CCM的不同蛋白质成分位于藻类细胞中的位置，然后我们将确定它们与哪些蛋白质相互作用，以及当CCM打开和关闭CCM时这些相互作用如何改变。这将使我们能够了解CCM的网络及其如何调节。最后，使用缺乏CCM单个蛋白质的突变体，我们将确定每个蛋白质成分的功能重要性。将组合本地化，交互和蛋白质功能数据，以创建CCM的详细3D地图，可以通过开放访问，在线交互式查看平台轻松探索该图。预计这些数据将有助于将CCM转移到作物植物中，以增加光合作用和产量。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts.

DOI：
10.7554/elife.37960
发表时间：
2018-10-11
期刊：
eLife
影响因子：
7.7
作者：
Küken A;Sommer F;Yaneva-Roder L;Mackinder LC;Höhne M;Geimer S;Jonikas MC;Schroda M;Stitt M;Nikoloski Z;Mettler-Altmann T
通讯作者：
Mettler-Altmann T

A recombineering pipeline to clone large and complex genes in Chlamydomonas

克隆衣藻中大型复杂基因的重组工程管道

DOI：
10.1101/2020.05.06.080416
发表时间：
2020
期刊：
影响因子：
0
作者：
Emrich-Mills T
通讯作者：
Emrich-Mills T

A recombineering pipeline to clone large and complex genes in Chlamydomonas.

DOI：
10.1093/plcell/koab024
发表时间：
2021-05-31
期刊：
The Plant cell
影响因子：
0
作者：
Emrich-Mills TZ;Yates G;Barrett J;Girr P;Grouneva I;Lau CS;Walker CE;Kwok TK;Davey JW;Johnson MP;Mackinder LCM
通讯作者：
Mackinder LCM

Endogenous GFP tagging in the diatom Thalassiosira pseudonana

硅藻假微型海链藻中的内源 GFP 标记