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Creating a spatially defined, multidimensional, protein interactome of the eukaryotic algal CO2 concentrating mechanism

创建真核藻类 CO2 浓缩机制的空间定义、多维蛋白质相互作用组

基本信息

批准号：
BB/R001014/1
负责人：
Luke Mackinder
金额：
$ 68.18万
依托单位：
University of York
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR001014%2F1
关键词：
Creating spatially defined multidimensional protein

项目摘要

Photosynthesis harnesses energy from the sun to fix carbon dioxide (CO2) into sugars and the protein building blocks of life. To enhance photosynthesis some plants and nearly all algae have evolved mechanisms to increase the accumulation of CO2 from their surrounding environment, this process in known as a CO2 concentrating mechanism (CCM). It is predicted that the transfer of a CCM to crop plants, such as rice and wheat that have failed to evolve CCMs, could increase yields by up to 60%. A promising CCM donor candidate is a green alga called Chlamydomonas, which has a highly efficient CCM. For the successful implementation of an algal CCM into crop plants it is essential that all the components are known, where they are found in the cell and how they function together. This project aims to rapidly identify all the proteins that make up the Chlamydomonas CCM and to determine how they interact with each other to form a functional unit that enhances CO2 uptake. To achieve this, we will determine where the different protein components of the CCM are located in the algal cell, we will then identify what proteins they are interacting with and how these interactions change when the CCM is switched on and off. This will allow us to understand the network of the CCM and how it is regulated. Finally, using mutants that lack individual proteins of the CCM, we will determine the functional importance of each protein component. The localisation, interaction and protein function data will be combined to create a detailed 3D map of the CCM that can be easily explored through an open-access, online interactive viewing platform. It is anticipated that these data will facilitate the transfer of a CCM into crop plants to increase photosynthesis and yields.

光合作用利用来自太阳的能量将二氧化碳（CO2）固定为糖和蛋白质，构成生命的基石。为了增强光合作用，一些植物和几乎所有的藻类都进化出了增加周围环境中CO2积累的机制，这个过程被称为CO2浓缩机制（CCM）。据预测，将CCM转移到作物植物中，如未能进化出CCM的水稻和小麦，产量可提高60%。一种有希望的CCM供体候选物是一种称为衣原体的绿色植物，其具有高效的CCM。为了将藻类CCM成功应用于作物植物，必须知道所有成分，它们在细胞中的位置以及它们如何共同发挥作用。该项目旨在快速识别构成衣原体CCM的所有蛋白质，并确定它们如何相互作用以形成增强CO2吸收的功能单元。为了实现这一目标，我们将确定CCM的不同蛋白质组分在藻类细胞中的位置，然后我们将确定它们与哪些蛋白质相互作用，以及当CCM打开和关闭时这些相互作用如何变化。这将使我们能够了解CCM的网络以及如何对其进行监管。最后，使用缺乏CCM的单个蛋白质的突变体，我们将确定每个蛋白质组分的功能重要性。定位，相互作用和蛋白质功能数据将结合起来，创建一个详细的CCM 3D地图，可以通过一个开放访问的在线交互式查看平台轻松探索。预计这些数据将有助于将CCM转移到作物植物中，以增加光合作用和产量。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Endogenous GFP tagging in the diatom Thalassiosira pseudonana

硅藻假微型海链藻中的内源 GFP 标记

DOI：
10.1101/2022.09.30.510313
发表时间：
2022
期刊：
影响因子：
0
作者：
Nam O
通讯作者：
Nam O

Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts.

DOI：
10.7554/elife.37960
发表时间：
2018-10-11
期刊：
eLife
影响因子：
7.7
作者：
Küken A;Sommer F;Yaneva-Roder L;Mackinder LC;Höhne M;Geimer S;Jonikas MC;Schroda M;Stitt M;Nikoloski Z;Mettler-Altmann T
通讯作者：
Mettler-Altmann T

A recombineering pipeline to clone large and complex genes in Chlamydomonas.

DOI：
10.1093/plcell/koab024
发表时间：
2021-05-31
期刊：
The Plant cell
影响因子：
0
作者：
Emrich-Mills TZ;Yates G;Barrett J;Girr P;Grouneva I;Lau CS;Walker CE;Kwok TK;Davey JW;Johnson MP;Mackinder LCM
通讯作者：
Mackinder LCM

A recombineering pipeline to clone large and complex genes in Chlamydomonas

克隆衣藻中大型复杂基因的重组工程管道