INTESTINAL LIPOPROTEIN METABOLISM

肠道脂蛋白代谢

• 批准号: 3235143
• 负责人: ALLEN COOPER
• 金额: $ 16.53万
• 依托单位: PALO ALTO MEDICAL FOUNDATION RES INST
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3235143
• 关键词: HMG coA reductases; Pongidae; apolipoproteins; atherosclerosis; blood lipoprotein metabolism; cholesterol; dietary lipid; enzyme mechanism; gastrointestinal neoplasms; high density lipoproteins; histochemistry /cytochemistry; human tissue; intestines; laboratory mouse; laboratory rabbit; laboratory rat; liver circulation; low density lipoprotein; nucleic acid probes; receptor; species difference

In this application, we propose to study several aspects of intestinal lipoprotein metabolism. First, we will evaluate to in depth the role of the various parts of the intestine in the degradation of low density lipoprotein (LDL). A unique aspect of this work will be the use of antireceptor antibodies to study LDL metabolism. The distribution of receptors along the GI tract will be determined by immunohistochemistry and direct binding. The presence of receptors in crypt and villous cells and the subcellular location of the receptors will also be determined. Regulation of receptor number under the influence of altered serum level and dietary and pharmacologic perturbation will be studied as well as the effect of malignant degeneration on receptor number. In other studies, the role of the LDL receptor in high density lipoprotein metabolism will be studied. The question of whether there is transport of cholesterol in excess of apoprotein will be addressed.

在本申请中，我们提出研究以下几个方面： 肠脂蛋白代谢 首先，我们将评估 在这一过程中，肠道的各个部分都发挥着重要作用。 低密度脂蛋白（LDL）降解。 的一个独特方面 本工作将使用抗受体抗体来研究LDL 新陈代谢. 受体沿着胃肠道的分布将 通过免疫组织化学和直接结合测定。 的 存在于隐窝和绒毛细胞和亚细胞的受体 还将确定受体的位置。 调控 受血清水平变化影响的受体数量， 饮食和药理干扰将进行研究，以及 恶性变性对受体数量的影响。 在其他研究中，低密度脂蛋白受体在高密度脂蛋白中的作用， 将研究脂蛋白代谢。 的问题 有运输胆固醇过量的载脂蛋白将 处理。