INTESTINAL LIPOPROTEIN METABOLISM

肠道脂蛋白代谢

• 批准号: 3235142
• 负责人: ALLEN COOPER
• 金额: $ 17.31万
• 依托单位: PALO ALTO MEDICAL FOUNDATION RES INST
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3235142
• 关键词: HMG coA reductases; Pongidae; apolipoproteins; atherosclerosis; blood lipoprotein metabolism; cholesterol; dietary lipid; enzyme mechanism; gastrointestinal neoplasms; high density lipoproteins; histochemistry /cytochemistry; human tissue; intestines; laboratory mouse; laboratory rabbit; laboratory rat; liver circulation; low density lipoprotein; nucleic acid probes; receptor; species difference

In this application, we propose to study several aspects of intestinal lipoprotein metabolism. First, we will evaluate to in depth the role of the various parts of the intestine in the degradation of low density lipoprotein (LDL). A unique aspect of this work will be the use of antireceptor antibodies to study LDL metabolism. The distribution of receptors along the GI tract will be determined by immunohistochemistry and direct binding. The presence of receptors in crypt and villous cells and the subcellular location of the receptors will also be determined. Regulation of receptor number under the influence of altered serum level and dietary and pharmacologic perturbation will be studied as well as the effect of malignant degeneration on receptor number. In other studies, the role of the LDL receptor in high density lipoprotein metabolism will be studied. The question of whether there is transport of cholesterol in excess of apoprotein will be addressed.

在本申请中，我们建议研究以下几个方面 肠道脂蛋白代谢。首先，我们将评估到In 肠的不同部分在肠道中的作用 低密度脂蛋白(LDL)的降解。的一个独特的方面 这项工作将利用抗受体抗体来研究低密度脂蛋白 新陈代谢。受体沿胃肠道的分布将 通过免疫组织化学和直接结合的方法确定。这个 隐窝和绒毛细胞及亚细胞中受体的存在 受体的位置也将被确定。规管 血药浓度变化对受体数量的影响及意义 还将研究饮食和药物方面的干扰以及 癌变对受体数量的影响。 在其他研究中，低密度脂蛋白受体在高密度脂蛋白中的作用 将对脂蛋白代谢进行研究。问题是，是否 有超过载脂蛋白的胆固醇的运输 地址。