EFFECTS OF OXALYL THIOESTERS ON ENZYMES

草酰硫酯对酶的影响

• 批准号: 3236369
• 负责人: GORDON A HAMILTON
• 金额: $ 7.96万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3236369
• 关键词: adenosinetriphosphatase; biotransformation; catalyst; fructose biphosphatase; glyceraldehyde 3 phosphate dehydrogenase; hexokinase; hormone regulation /control mechanism; insulin; isocitrate dehydrogenase; malate dehydrogenase; oncogenes; oxalates; physical chemical interaction; pyruvate kinase; thioester

Several lines of research in this laboratory have recently converged to suggest that oxalyl thioesters (RSCOCOO-) may plan an important role in controlling metabolism in animals, and may function as mediators for some hormones, especially insulin. If oxalyl thioesters are functioning in this way then obviously they must modify the catalytic activities of various enzymes, and preliminary results with a few systems indicate that to be the case. In order to evaluate how important a system of metabolic control based on oxalyl thioesters might be, it will be necessary to characterize more thoroughly their effects on these as well as other enzymes. That is the general goal of the proposed research. The effects of oxalyl thioesters on several of the following animal enzymes will be investigated: protein phosphatases, malic enzyme, succinyl CoA transferase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, hexokinase, fructose-1,6-diphosphatase, isocitrate dehydrogenase, Na/K ATPase, etc. Initially the effect of oxalyl thioester structure, concentration and time of incubation with enzyme will be studied but eventually the detailed mechanism for any enzyme modifications will be investigated. The research potentially could lead to the elucidation of a whole new system of metabolic control in animals. Not only may it be particularly relevant to the insulin related disease, diabetes, but also to diseases such as cancer, since various growth factors and protein products of oncogenes seem closely related to insulin.

这个实验室的几个研究方向最近都集中在 这表明草酰硫酯（RSCOCOO-）可能在 控制动物的新陈代谢，并可能作为一些 激素，尤其是胰岛素。 如果草酰硫酯在这一过程中起作用， 显然，它们必须改变各种催化剂的催化活性， 一些系统的初步结果表明， 案子 为了评估代谢控制系统 基于草酰硫酯可能是，这将是必要的，以表征 更彻底地研究它们对这些酶以及其他酶的影响。 即 拟议研究的总体目标。 草酰硫酯对以下几种动物酶的影响 将研究：蛋白磷酸酶，苹果酸酶，琥珀酰辅酶A 转移酶，甘油醛-3-磷酸脱氢酶，丙酮酸激酶， 己糖激酶、果糖-1，6-二磷酸酶、异柠檬酸脱氢酶、Na/K ATP酶等。最初草酰硫酯结构的影响， 将研究与酶孵育的浓度和时间， 最终，任何酶修饰的详细机制将是 研究了 这项研究可能会导致一个全新的解释， 动物的代谢控制系统。 它不仅可能特别 与胰岛素相关的疾病，糖尿病， 例如癌症，因为各种生长因子和蛋白质产物， 癌基因似乎与胰岛素密切相关。