EFFECTS OF OXALYL THIOESTERS ON ENZYMES

这个实验室的几个研究方向最近汇聚到 提示草酰硫代酯(RSCOCOO-)可能在 控制动物的新陈代谢，并可能在某些情况下发挥中介作用 激素，尤其是胰岛素。如果草酰硫代酯在这个过程中起作用 那么显然他们必须改变各种不同的催化活性 酶，和一些系统的初步结果表明，这是 凯斯。为了评估代谢控制系统的重要性 基于草酰基硫酸酯的可能，有必要对其进行表征 更彻底地影响这些酶和其他酶。那是 拟议研究的总体目标。 草酰硫酸酯对以下几种动物酶的影响 将进行研究：蛋白磷酸酶、苹果酸酶、琥珀酰辅酶A 转移酶，甘油醛-3-磷酸脱氢酶，丙酮酸激酶， 己糖激酶、果糖-1，6-二磷酸酶、异柠檬酸脱氢酶、钠/钾 ATPase等。初步探讨了草酰硫酯结构的影响， 与酶孵育的浓度和时间将被研究，但 最终，任何酶修饰的详细机制都将是 调查过了。 这项研究可能会导致对一种全新的 动物新陈代谢控制系统。不仅可能是特别的 与胰岛素相关的疾病，糖尿病，但也与疾病有关 例如癌症，因为各种生长因子和蛋白质产品 癌基因似乎与胰岛素密切相关。

Several lines of research in this laboratory have recently converged to suggest that oxalyl thioesters (RSCOCOO-) may plan an important role in controlling metabolism in animals, and may function as mediators for some hormones, especially insulin. If oxalyl thioesters are functioning in this way then obviously they must modify the catalytic activities of various enzymes, and preliminary results with a few systems indicate that to be the case. In order to evaluate how important a system of metabolic control based on oxalyl thioesters might be, it will be necessary to characterize more thoroughly their effects on these as well as other enzymes. That is the general goal of the proposed research. The effects of oxalyl thioesters on several of the following animal enzymes will be investigated: protein phosphatases, malic enzyme, succinyl CoA transferase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, hexokinase, fructose-1,6-diphosphatase, isocitrate dehydrogenase, Na/K ATPase, etc. Initially the effect of oxalyl thioester structure, concentration and time of incubation with enzyme will be studied but eventually the detailed mechanism for any enzyme modifications will be investigated. The research potentially could lead to the elucidation of a whole new system of metabolic control in animals. Not only may it be particularly relevant to the insulin related disease, diabetes, but also to diseases such as cancer, since various growth factors and protein products of oncogenes seem closely related to insulin.