EFFECTS OF OXALYL THIOESTERS ON ENZYMES
EFFECTS OF OXALYL THIOESTERS ON ENZYMES
批准号:
3236370
负责人:
GORDON A HAMILTON
金额:
$8.31万
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-07-01 至 1989-06-30
关键词:
adenosinetriphosphatase biotransformation catalyst enzyme mechanism fructose biphosphatase glyceraldehyde 3 phosphate dehydrogenase hexokinase hormone regulation /control mechanism insulin isocitrate dehydrogenase malate dehydrogenase oncogenes oxalates physical chemical interaction pyruvate kinase sodium potassium exchanging ATPase thioester
中文摘要
这个实验室的几个研究方向最近汇聚到
提示草酰硫代酯(RSCOCOO-)可能在
控制动物的新陈代谢,并可能在某些情况下发挥中介作用
激素,尤其是胰岛素。如果草酰硫代酯在这个过程中起作用
那么显然他们必须改变各种不同的催化活性
酶,和一些系统的初步结果表明,这是
凯斯。为了评估代谢控制系统的重要性
基于草酰基硫酸酯的可能,有必要对其进行表征
更彻底地影响这些酶和其他酶。那是
拟议研究的总体目标。
草酰硫酸酯对以下几种动物酶的影响
将进行研究:蛋白磷酸酶、苹果酸酶、琥珀酰辅酶A
转移酶,甘油醛-3-磷酸脱氢酶,丙酮酸激酶,
己糖激酶、果糖-1,6-二磷酸酶、异柠檬酸脱氢酶、钠/钾
ATPase等。初步探讨了草酰硫酯结构的影响,
与酶孵育的浓度和时间将被研究,但
最终,任何酶修饰的详细机制都将是
调查过了。
这项研究可能会导致对一种全新的
动物新陈代谢控制系统。不仅可能是特别的
与胰岛素相关的疾病,糖尿病,但也与疾病有关
例如癌症,因为各种生长因子和蛋白质产品
癌基因似乎与胰岛素密切相关。
英文摘要
Several lines of research in this laboratory have recently converged to
suggest that oxalyl thioesters (RSCOCOO-) may plan an important role in
controlling metabolism in animals, and may function as mediators for some
hormones, especially insulin. If oxalyl thioesters are functioning in this
way then obviously they must modify the catalytic activities of various
enzymes, and preliminary results with a few systems indicate that to be the
case. In order to evaluate how important a system of metabolic control
based on oxalyl thioesters might be, it will be necessary to characterize
more thoroughly their effects on these as well as other enzymes. That is
the general goal of the proposed research.
The effects of oxalyl thioesters on several of the following animal enzymes
will be investigated: protein phosphatases, malic enzyme, succinyl CoA
transferase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase,
hexokinase, fructose-1,6-diphosphatase, isocitrate dehydrogenase, Na/K
ATPase, etc. Initially the effect of oxalyl thioester structure,
concentration and time of incubation with enzyme will be studied but
eventually the detailed mechanism for any enzyme modifications will be
investigated.
The research potentially could lead to the elucidation of a whole new
system of metabolic control in animals. Not only may it be particularly
relevant to the insulin related disease, diabetes, but also to diseases
such as cancer, since various growth factors and protein products of
oncogenes seem closely related to insulin.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
S-oxalylglutathione is a substrate for gamma-glutamyltransferase (GGT). Implications for the role of GGT in cell proliferation.
S-草酰谷胱甘肽是 γ-谷氨酰转移酶 (GGT) 的底物。
DOI:
10.1016/0006-291x(88)90466-4
发表时间:
1988
期刊:
Biochemical and biophysical research communications
影响因子:
3.1
作者:
[Hamilton,GA, Buckthal,DJ, Kantorczyk,NJ, Skorczynski,SS]
通讯作者:
Skorczynski,SS
OXALINS AND CELL PROLIFERATION AND REGULATION
-
批准号:2183219
-
项目类别:
-
资助金额:$17.81万
-
财政年份:1993
-
负责人:GORDON A HAMILTON
-
依托单位:
OXALINS AND CELL PROLIFERATION AND REGULATION
-
批准号:3304958
-
项目类别:
-
资助金额:$19.15万
-
财政年份:1993
-
负责人:GORDON A HAMILTON
-
依托单位:
OXALINS AND CELL PROLIFERATION AND REGULATION
-
批准号:2183220
-
项目类别:
-
资助金额:$18.38万
-
财政年份:1993
-
负责人:GORDON A HAMILTON
-
依托单位:
ROLE OF OXALINS IN CELL PROLIFERATION AND REGULATION
-
批准号:3509796
-
项目类别:
-
资助金额:$10.0万
-
财政年份:1991
-
负责人:GORDON A HAMILTON
-
依托单位:
ENZYMES IN ANIMAL METABOLISM OF OXALATE DERIVATIVES
-
批准号:3238056
-
项目类别:
-
资助金额:$13.38万
-
财政年份:1988
-
负责人:GORDON A HAMILTON
-
依托单位:
ENZYMES IN ANIMAL METABOLISM OF OXALATE DERIVATIVES
-
批准号:3238054
-
项目类别:
-
资助金额:$14.07万
-
财政年份:1988
-
负责人:GORDON A HAMILTON
-
依托单位:
ENZYMES IN ANIMAL METABOLISM OF OXALATE DERIVATIVES
-
批准号:3238055
-
项目类别:
-
资助金额:$12.39万
-
财政年份:1988
-
负责人:GORDON A HAMILTON
-
依托单位:
EFFECTS OF OXALYL THIOESTERS ON ENZYMES
-
批准号:3236368
-
项目类别:
-
资助金额:$7.84万
-
财政年份:1986
-
负责人:GORDON A HAMILTON
-
依托单位:
EFFECTS OF OXALYL THIOESTERS ON ENZYMES
-
批准号:3236369
-
项目类别:
-
资助金额:$7.96万
-
财政年份:1986
-
负责人:GORDON A HAMILTON
-
依托单位:
A MECHANISTIC STUDY OF SOME O2 OXIDOREDUCTASES
-
批准号:3225052
-
项目类别:
-
资助金额:$11.86万
-
财政年份:1977
-
负责人:GORDON A HAMILTON
-
依托单位:
A MECHANISTIC STUDY OF SOME O2 OXIDOREDUCTASES
-
批准号:3150867
-
项目类别:
-
资助金额:$17.21万
-
财政年份:1977
-
负责人:GORDON A HAMILTON
-
依托单位:
A MECHANISTIC STUDY OF SOME O2 OXIDOREDUCTASES
-
批准号:3225050
-
项目类别:
-
资助金额:$17.23万
-
财政年份:1977
-
负责人:GORDON A HAMILTON
-
依托单位:
A MECHANISTIC STUDY OF SOME O2 OXIDOREDUCTASES
-
批准号:3225054
-
项目类别:
-
资助金额:$13.72万
-
财政年份:1977
-
负责人:GORDON A HAMILTON
-
依托单位:
A MECHANISTIC STUDY OF SOME O2 OXIDOREDUCTASES
-
批准号:3225051
-
项目类别:
-
资助金额:$10.99万
-
财政年份:1977
-
负责人:GORDON A HAMILTON
-
依托单位:
A MECHANISTIC STUDY OF SOME O2 OXIDOREDUCTASES
-
批准号:3225053
-
项目类别:
-
资助金额:$13.25万
-
财政年份:1977
-
负责人:GORDON A HAMILTON
-
依托单位:
A MECHANISTIC STUDY OF SOME O2 OXIDOREDUCTASES
-
批准号:3225049
-
项目类别:
-
资助金额:$13.09万
-
财政年份:1977
-
负责人:GORDON A HAMILTON
-
依托单位:
海外基金