BENZENE TOXICITY AND METABOLISM

这项建议的总目标仍然是研究 苯代谢与苯毒性的关系。 在这 在此期间，我们希望研究苯与DNA的共价结合 使用分离的酶和细胞系统来产生生物反应性 苯和添加的DNA的中间体。 然后我们将比较加合物 在这些系统中形成的与那些由苯的管理形成的 in vivo. 体内形成的加合物将使用“后加合物”测量。 标记测定”。 我们还计划研究苯对细胞生产的影响， 生长因子 因此，我们将确定苯及其 代谢物对T淋巴细胞产生造血细胞的能力的影响 因子IL-3（multiCSA）和粒细胞/巨噬细胞集落刺激 活动（GM-CSA）。 我们还将确定苯对 单核细胞介导的造血因子的产生和/或释放 T淋巴细胞

The general aim of this proposal continues to be the study of the relationship between benzene metabolism and benzene toxicity. In this grant period we wish to investigate the covalent binding of benzene to DNA using isolated enzyme and cell systems to produce biological reactive intermediates of benzene and added DNA. We will then compare the adducts formed in these systems with those formed by the administration of benzene in vivo. The adducts formed in vivo will be measured using the "post labeling assay". We also plan to study the effects of benzene on the production of cell growth factors. Thus, we will determine the effects of benzene and its metabolites on the ability of the T-lymphocyte to produce the hematopoietic factors IL-3(multiCSA) and granulocyte/macrophage colony stimulating activity (GM-CSA). We will also determine the effects of benzene on the monocyte-directed production and/or release of hematopoietic factors by the T-lymphocyte.